PLASMID DNA DAMAGE INDUCED BY SINGLET MOLECULAR OXYGEN RELEASED FROM THE NAPHTHALENE ENDOPEROXIDE DHPNO2 AND PHOTOACTIVATED METHYLENE BLUE

被引：13

作者：

Berra, Carolina Maria ^{[1
]}

Martins Menck, Carlos Frederico ^{[1
]}

Martinez, Glaucia Regina ^{[2
]}

de Oliveira, Carla Santos ^{[3
]}

Baptista, Mauricio da Silva ^{[4
]}

Di Mascio, Paolo ^{[4
]}

机构：

[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo, Brazil

[2] Univ Fed Parana, Setor Ciencias Biol, Dept Bioquim & Biol Mol, BR-81531990 Curitiba, Parana, Brazil

[3] Univ Fed Mato Grosso do Sul, Ctr Ciencias Biol & Saude, Dept Morfofisiol, BR-79070900 Campo Grande, MS, Brazil

[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05599970 Sao Paulo, Brazil

来源：

QUIMICA NOVA | 2010年 / 33卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

oxidative stress; DNA lesions; singlet molecular oxygen; ESCHERICHIA-COLI; SUBSTRATE-SPECIFICITY; MUTAGENIC SUBSTRATE; FPG PROTEIN; REPAIR; OXIDATION; GLYCOSYLASE; GENOTOXICITY; PROFLAVINE; GENERATION;

D O I：

10.1590/S0100-40422010000200009

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

PLASMID DNA DAMAGE INDUCED BY SINGLET MOLECULAR OXYGEN RELEASED FROM THE NAPHTHALENE ENDOPEROXIDE DHPNO2 AND PHOTOACTIVATED METHYLENE BLUE. To investigate oxidative lesions and strand breaks induction by singlet Molecular oxygen (O-1(2)), supercoiled-DNA plasmid was treated with thermo-dissociated DHPNO2 and photoactivated-methylene blue. DNA lesions were detected by Fpg that cleaves DNA at certain oxidized bases, and T4-endoV. which cleaves DNA at cyclobutane pyrimidine dimers and apurinic/apyrimidinic (AP) sites. These cleavages form open relaxed-DNA structures, which are discriminated from supercoiled-DNA. DHPNO2 or photoactivated-MB treatments result in similar plasmid damage profile: low number of single-strand breaks or AP-sites and high frequency of Fpg-sensitive sites; confirming that base oxidation is the main product for both reactions and that O-1(2) might be the most likely intermediate that reacts with DNA.

引用

页码：279 / 283

页数：5

共 41 条

[1] SINGLET OXYGEN INDUCES FRANK STRAND BREAKS AS WELL AS ALKALI-LABILE AND PIPERIDINE-LABILE SITES IN SUPERCOILED PLASMID DNA [J].

BLAZEK, ER ;

PEAK, JG ;

PEAK, MJ .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1989, 49 (05) :607-613

[2] SUBSTRATE-SPECIFICITY OF THE ESCHERICHIA-COLI FPG PROTEIN (FORMAMIDOPYRIMIDINE DNA GLYCOSYLASE) - EXCISION OF PURINE LESIONS IN DNA PRODUCED BY IONIZING-RADIATION OR PHOTOSENSITIZATION [J].

BOITEUX, S ;

GAJEWSKI, E ;

LAVAL, J ;

DIZDAROGLU, M .

BIOCHEMISTRY, 1992, 31 (01) :106-110

[3] FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE OF ESCHERICHIA-COLI - CLONING AND SEQUENCING OF THE FPG STRUCTURAL GENE AND OVERPRODUCTION OF THE PROTEIN [J].

BOITEUX, S ;

OCONNOR, TR ;

LAVAL, J .

EMBO JOURNAL, 1987, 6 (10) :3177-3183

[4] SINGLET OXYGEN FORMATION DETECTED BY LOW-LEVEL CHEMI-LUMINESCENCE DURING ENZYMATIC REDUCTION OF PROSTAGLANDIN-G2 TO PROSTAGLANDIN-H2 [J].

CADENAS, E ;

SIES, H ;

NASTAINCZYK, W ;

ULLRICH, V .

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1983, 364 (05) :519-528

[5]

Cadet J, 2000, METHOD ENZYMOL, V319, P143

[6] Singlet oxygen oxidation of isolated and cellular DNA: Product formation and mechanistic insights [J].

Cadet, Jean ;

Ravanat, Jean-Luc ;

Martinez, Glaucia R. ;

Medeiros, Mansa H. G. ;

Di Mascio, Paolo .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2006, 82 (05) :1219-1225

[7] Cytotoxicity and mutagenesis induced by singlet oxygen in wild type and DNA repair deficient Escherichia coli strains [J].

Cavalcante, AKD ;

Martinez, GR ;

Di Mascio, P ;

Menck, CFM ;

Agnez-Lima, LF .

DNA REPAIR, 2002, 1 (12) :1051-1056

[8]

Dewilde A, 1998, BIOL CHEM, V379, P1377

[9]

DI MASCIO P, 1990, PHOTOCHEM PHOTOBIOL, V51, P293

[10] Repair and mutagenic potential of oxaluric acid, a major product of singlet oxygen-mediated oxidation of 8-oxo-7,8-dihydroguanine [J].

Duarte, V ;

Gasparutto, D ;

Jaquinod, M ;

Ravanat, JL ;

Cadet, J .

CHEMICAL RESEARCH IN TOXICOLOGY, 2001, 14 (01) :46-53

← 1 2 3 4 5 →