Investigating endothelial invasion and sprouting behavior in three-dimensional collagen matrices

被引：91

作者：

Bayless, Kayla J. ^{[1
]}

Kwak, Hyeong-Il ^{[1
]}

Su, Shih-Chi ^{[1
]}

机构：

[1] Texas A&M Hlth Sci Ctr, Dept Mol & Cellular Med, College Stn, TX 77843 USA

来源：

NATURE PROTOCOLS | 2009年 / 4卷 / 12期

关键词：

DIFFERENTIAL GENE-EXPRESSION; CAPILLARY LUMEN FORMATION; IN-VITRO; CELL INVASION; EXTRACELLULAR-MATRIX; G-PROTEIN; REQUIRES; ANGIOGENESIS; FIBROBLASTS; MORPHOGENESIS;

D O I：

10.1038/nprot.2009.221

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Seeding a monolayer of primary human endothelial cells on the surface of a polymerized three-dimensional collagen matrix in the presence of pro-angiogenic stimuli allows manipulation and analysis of rapid sprouting responses. This protocol is useful for elucidating incompletely defined intracellular mechanisms downstream of pro-angiogenic factors that regulate sprout formation and initiation, and can also be used to test the efficacy of pro-and anti-angiogenic compounds. We present protocols to culture endothelial cells, prepare three-dimensional collagen matrices and quantify and image rapid endothelial sprouting responses (24 h). This protocol can be carried out using either type I or type II collagen matrices with primary endothelial cells isolated from macrovascular and microvascular sources of varying species.

引用

页码：1888 / 1898

页数：11

共 49 条

[1] The aortic ring model of angiogenesis [J].

Aplin, Alfred C. ;

Fogel, Eric ;

Zorzi, Penelope ;

Nicosia, Roberto F. .

ANGIOGENESIS: IN VITRO SYSTEMS, 2008, 443 :119-136

[2] Sphingosine-1-phosphate markedly induces matrix metalloproteinase and integrin-dependent human endothelial cell invasion and lumen formation in three-dimensional collagen and fibrin matrices [J].

Bayless, KJ ;

Davis, GE .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 312 (04) :903-913

[3]

Bayless KJ, 2002, J CELL SCI, V115, P1123

[4]

Bell SE, 2001, J CELL SCI, V114, P2755

[5] PHENOTYPIC MODULATIONS OF HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS AND HUMAN DERMAL FIBROBLASTS USING 2 ANGIOGENIC ASSAYS [J].

BIKFALVI, A ;

CRAMER, EM ;

TENZA, D ;

TOBELEM, G .

BIOLOGY OF THE CELL, 1991, 72 (03) :275-278

[6]

BORNSTEIN MB, 1958, LAB INVEST, V7, P134

[7]

Davis GE, 2000, IN VITRO CELL DEV-AN, V36, P513

[8] An alpha 2 beta 1 integrin-dependent pinocytic mechanism involving intracellular vacuole formation and coalescence regulates capillary lumen and tube formation in three-dimensional collagen matrix [J].

Davis, GE ;

Camarillo, CW .

EXPERIMENTAL CELL RESEARCH, 1996, 224 (01) :39-51

[9] Progesterone and Placentation Increase Secreted Phosphoprotein One (SPP1 or Osteopontin) in Uterine Glands and Stroma for Histotrophic and Hematotrophic Support of Ovine Pregnancy [J].

Dunlap, Kathrin A. ;

Erikson, David W. ;

Burghardt, Robert C. ;

White, Frank J. ;

Reed, Kristey M. ;

Farmer, Jennifer L. ;

Spencer, Thomas E. ;

Magness, Ronald R. ;

Bazer, Fuller W. ;

Bayless, Kayla J. ;

Johnson, Greg A. .

BIOLOGY OF REPRODUCTION, 2008, 79 (05) :983-990

[10] Visualizing stromal cell dynamics in different tumor microenvironments by spinning disk confocal microscopy [J].

Egeblad, Mikala ;

Ewald, Andrew J. ;

Askautrud, Hanne A. ;

Truitt, Morgan L. ;

Welm, Bryan E. ;

Bainbridge, Emma ;

Peeters, George ;

Krummel, Matthew F. ;

Werb, Zena .

DISEASE MODELS & MECHANISMS, 2008, 1 (2-3) :155-167

← 1 2 3 4 5 →