Adrenomedullin promotes the odontogenic differentiation of dental pulp stem cells through CREB/BMP2 signaling pathway

被引：9

作者：

Zhu, Qiang ^{[1
]}

Gao, Jianyong ^{[1
]}

Tian, Gang ^{[1
]}

Tang, Zhen ^{[1
]}

Tan, Yinghui ^{[2
]}

机构：

[1] Second Mil Med Univ, Changhai Hosp, Dept Stomatol, Shanghai 200433, Peoples R China

[2] Third Mil Med Univ, Xinqiao Hosp, Dept Stomatol, Chongqing 400037, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2017年 / 49卷 / 07期

关键词：

adrenomedullin; dental pulp stem cell; odontogenic differentiation; BONE MORPHOGENETIC PROTEINS; ELEMENT-BINDING PROTEIN; PARATHYROID-HORMONE REQUIRES; CAMP RESPONSE ELEMENT; KINASE-A; GENE-EXPRESSION; TRANSCRIPTION; INDUCTION; PEPTIDE; BMP2;

D O I：

10.1093/abbs/gmx053

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adrenomedullin (AM) could promote the proliferation, the odontogenic differentiation and inhibit the apoptosis of dental pulp stem cells (DPSCs). AM in combination with DPSCs may be an effective strategy for pulp repair. However, there was no report on the mechanisms of AM in the odontogenic differentiation of DPSCs. The aim of this study is to investigate the molecular mechanisms through which AM promotes the odontogenic differentiation of DPSCs. Freshly extracted wisdom teeth were obtained from 27 patients. Cells at passage 3 to passage 5 were used in this study. DPSCs were treated with or without 10-7M AM in Dulbecco's modified Eagle's medium culture, and then the accumulated calcium deposition was analyzed after 21 days by using alizarin red S staining. Odontogenic differentiation markers were determined by western blot analysis and quantitative real-time PCR. Western blot analysis results showed that AM had the capability of promoting the odontogenic differentiation of DPSCs and AM could enhance the phosphorylation of CREB and up-regulate the expression of BMP2. H89 is a CREB inhibitor which can inhibit the odontogenic differentiation of DPSCs through inhibiting the phosphorylation of CREB. Noggin could inhibit the odontogenic differentiation of DPSCs through inhibiting the activity of BMP2. These results indicated that AM could promote the odontogenic differentiation of DPSCs by upregulating the expression of BMP2 through the CREB signaling pathway.

引用

页码：609 / 616

页数：8

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