TALE-triggered and iTALE-suppressed Xa1-mediated resistance to bacterial blight is independent of rice transcription factor subunits OsTFIIAγ1 or OsTFIIAγ5

被引：16

作者：

Xu, Xiameng ^{[1
,2
]}

Xu, Zhengyin ^{[1
,2
]}

Ma, Wenxiu ^{[1
,4
]}

Haq, Fazal ^{[1
,2
]}

Li, Ying ^{[1
,2
]}

Shah, Syed Mashab Ali ^{[1
,2
]}

Zhu, Bo ^{[2
]}

Zhu, Changxiang ^{[3
]}

Zou, Lifang ^{[2
]}

Chen, Gongyou ^{[1
,2
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Agr & Biol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Agr & Biol, Key Lab Urban Agr, Minist Agr China, Shanghai 200240, Peoples R China

[3] Shandong Agr Univ, Coll Life Sci, Natl Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

[4] Univ Florida, Citrus Res & Educ Ctr, Inst Food & Agr Sci, Lake Alfred, FL 33850 USA

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 2021年 / 72卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Bacterial blight; iTALE; OsTFIIA gamma 1; OsTFIIA gamma 5; TALE; Xa1-mediated resistance; DISEASE RESISTANCE; GENE; EXPRESSION; IDENTIFICATION; AVIRULENCE; RECOGNITION; EVOLUTION; EFFECTORS; HOST; XA1;

D O I：

10.1093/jxb/erab054

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Xa1-mediated resistance to rice bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is triggered by transcription activator-like effectors (TALEs) and suppressed by interfering TALEs (iTALEs). TALEs interact with the rice transcription factor OsTFIIA gamma 1 or OsTFIIA gamma 5 (Xa5) to activate expression of target resistance and/or susceptibility genes. However, it is not clear whether OsTFIIA gamma is involved in TALE-triggered and iTALE-suppressed Xa1-mediated resistance. In this study, genome-edited mutations in OsTFIIA gamma 5 or Os TFIIA gamma 1 of Xa1-containing rice 'IRBB1' and Xaltransgenic plants of xa5-containing rice 'IRBB5' did not impair the activation or suppression of Xa1-mediated resistance. Correspondingly, the expression pattern of Xa1 in mutated OsTFIIA gamma 5 and OsTFIIA gamma 1 rice lines and 'IRBB1' rice was similar. In contrast, the expression of OsSWEET11 was repressed in rice lines mutated in OsTFIIA gamma 5 and OsTFIIA gamma 1. Bimolecular fluorescence complementation (BiFC) and co-immunoprecipitation assays showed that both TALE PthXo1 and iTALE Tal3a interacted with OsTFIIAy1 and OsTFIIA gamma 5 in plant nuclei. These results indicated that TALE-triggered and iTALE-suppressed Xa1-mediated resistance to bacterial blight is independent of OsTFIIA gamma 1 or OsTFIIA gamma 5 in rice, and suggest that an unknown factor is potentially involved in the interaction of Xa1, TALEs and iTALEs.

引用

页码：3249 / 3262

页数：14

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