Direct quantification in bioanalytical LC-MS/MS using internal calibration via analyte/stable isotope ratio

被引:62
作者
Nilsson, Lars B. [1 ]
Eklund, Goran [1 ]
机构
[1] AstraZeneca R&D, Global Dev DMPK & Bioanal, Dev DMPK & Bioanal Sodertalje, SE-15185 Sodertalje, Sweden
关键词
stable isotope labeled internal standard; calibration curve; LC-MS/MS;
D O I
10.1016/j.jpba.2006.09.030
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The possibility to rationalize and simplify bioanalysis, without compromising the analytical quality, by omitting the calibration curves was studied. Using mass spectrometry (MS) and a stable isotope labeled internal standard it was possible to get equally good results by calculating the results directly from the analyte/intemal standard area ratio and a predetermined response factor as by the traditional way, using a calibration curve run at the same occasion. To be able to use this simplified quantification method, that we call internal calibration, in its most simple form there are some prerequisites that must be considered: (1) The relative response should not be concentration dependent. (2) The relative response should be constant between batches/days. (3) The level of analyte in the internal standard should not be detectable. (4) There should be no influence from naturally occurring isotopes of the analyte on the internal standard peak area. A bioanalytical LC-MS/MS method for a research compound was validated both with and without calibration curves and no significant differences were found regarding precision and accuracy. It was shown that all four prerequisites above were fulfilled. Validation data were very good for the whole concentration range, 0.010-30 mu mol/L. Long-term data for QC samples showed excellent precision and accuracy. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:1094 / 1099
页数:6
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