Detection of Crimean-Congo hemorrhagic fever virus genome in saliva and urine

被引:26
作者
Bodur, Hurrem [1 ]
Akinci, Esragul [1 ]
Onguru, Pinar [1 ]
Carhan, Ahmet [2 ]
Uyar, Yavuz [2 ]
Tanrici, Aliye [1 ]
Cataloluk, Osman [3 ]
Kubar, Ayhan [4 ]
机构
[1] Ankara Numune Training & Res Hosp, Dept Infect Dis & Clin Microbiol, TR-06100 Ankara, Turkey
[2] Refik Saydam Hifzissihha Ctr, Dept Virol, Ankara, Turkey
[3] Balikesir Univ, Dept Microbiol, Balikesir, Turkey
[4] Gulhane Mil Med Acad, Dept Virol, Ankara, Turkey
关键词
Crimean-Congo hemorrhagic fever; Saliva; Urine; CLINICAL-FEATURES; INFECTION; OUTBREAK; TURKEY;
D O I
10.1016/j.ijid.2009.04.018
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background: The Crimean-Congo hemorrhagic fever (CCHF) virus is transmitted by tick bites and by contact with the blood or tissues of infected patients and livestock. This study was designed to investigate the genome of CCHF virus in saliva and urine samples of patients with CCHF. Methods: Eight patients with laboratory-confirmed CCHF were included in the study. The diagnosis was made by detection of viral RNA in blood by real-time reverse transcriptase-polymerase chain reaction (real-time RT-PCR). Samples of saliva from six patients and samples of urine from three patients were collected at the same time as the blood samples and analyzed for viral RNA. Results: The genome of CCHF virus was detected in the saliva from five of the six patients and in the urine from two of the three patients. The levels of viral load in the saliva and urine samples were similar to those in the blood samples in all but one patient, in whom higher levels were detected in blood compared to saliva or urine. Conclusions: This study shows that during human infection with CCHF virus, viral genomes are present in the saliva and urine. Further studies to isolate infectious viruses from these fluids and to study whether they represent an infectious risk are underway. (C) 2009 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:E247 / E249
页数:3
相关论文
共 14 条
[1]  
[Anonymous], Crimean-Congo haemorrhagic fever
[2]   Crimean-Congo haemorrhagic fever outbreak in Middle Anatolia: a multicentre study of clinical features and outcome measures [J].
Bakir, M ;
Ugurlu, M ;
Dokuzoguz, B ;
Bodur, H ;
Tasyaran, MA ;
Vahaboglu, H .
JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 54 (04) :385-389
[3]   Immunodiagnosis of dengue virus infection using saliva [J].
Chakravarti, Anita ;
Matlani, Monika ;
Jain, Manisha .
CURRENT MICROBIOLOGY, 2007, 55 (06) :461-464
[4]   Characteristics of patients with Crimean-Congo hemorrhagic fever in a recent outbreak in Turkey and impact of oral ribavirin therapy [J].
Ergönül, Ö ;
Çelikbas, A ;
Dokuzoguz, B ;
Eren, S ;
Baykam, N ;
Esener, H .
CLINICAL INFECTIOUS DISEASES, 2004, 39 (02) :284-287
[5]  
Evika MA, 2008, INT J INFECT DIS, V12, P374, DOI [10.1016/j.ijid.2007.09.01, 10.1016/j.ijid.2007.09.010]
[6]  
Izadi S, 2006, JPN J INFECT DIS, V59, P326
[7]   DETECTION OF LASSA VIRUS-RNA IN SPECIMENS FROM PATIENTS WITH LASSA FEVER BY USING THE POLYMERASE CHAIN-REACTION [J].
LUNKENHEIMER, K ;
HUFERT, FT ;
SCHMITZ, H .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (12) :2689-2692
[8]   Confirmation of dengue virus infection by detection of dengue virus type 1 genome in urine and saliva but not in plasma [J].
Mizuno, Y. ;
Kotaki, A. ;
Harada, F. ;
Tajima, S. ;
Kurane, I. ;
Takasaki, T. .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2007, 101 (07) :738-739
[9]   Crimean-Congo hemorrhagic fever in Eastern Turkey: clinical features, risk factors and efficacy of ribavirin therapy [J].
Ozkurt, Z ;
Kiki, H ;
Erol, S ;
Erdem, F ;
Yilmaz, N ;
Partak, M ;
Gundogdu, M ;
Tasyaran, MA .
JOURNAL OF INFECTION, 2006, 52 (03) :207-215
[10]   ARGENTINE HEMORRHAGIC-FEVER - EARLY DIAGNOSIS BY IMMUNOFLUORESCENCE [J].
RUGGIERO, HA ;
MILANI, HL ;
MAGNONI, C ;
MILANI, HA ;
FRIGERIO, M .
ARCHIVES OF VIROLOGY, 1981, 70 (02) :165-168