Tyrosinase based biosensor for the electrochemical determination of sulfamethoxazole

被引：43

作者：

del Torno-de Roman, Lorena ^{[1
]}

Asuncion Alonso-Lomillo, M. ^{[1
]}

Dominguez-Renedo, Olga ^{[1
]}

Julia Arcos-Martinez, M. ^{[1
]}

机构：

[1] Univ Burgos, Dept Analyt Chem, Fac Sci, Pza Misael Banuelos S-N, Burgos 09001, Spain

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2016年 / 227卷

关键词：

Biosensor; Screen-printed carbon electrodes; Gold nanoparticles; Tyrosinase Sulfamethoxazole; SOLID-PHASE EXTRACTION; PULSE VOLTAMMETRIC DETERMINATION; PERFORMANCE LIQUID-CHROMATOGRAPHY; CAPILLARY-ZONE-ELECTROPHORESIS; DOPED DIAMOND ELECTRODE; POTENTIOMETRIC TRANSDUCTION; WATER SAMPLES; WASTE-WATER; SULFONAMIDE RESIDUES; CATALYTIC-OXIDATION;

D O I：

10.1016/j.snb.2015.12.053

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A disposable amperometric biosensor has been developed for the determination of sulfamethoxazole (SMX). Tyrosinase (TYR) has been cross -linked to screen-printed carbon electrodes previously modified by gold nanoparticles. The oxidation current recorded at +500 mV vs Ag/AgCl SPE has been related to SMX concentration. The biosensor showed an acceptable inter and intra immobilization assay, with a RSD of 5.8% (n = 4) and 6.7% (n = 4), respectively, in the concentration range from 20 mu m to 0.2 mM. The capability of detection was 22.6 +/- 2.1 OA for a probability of false positive and negative of 0.05. Finally, the developed biosensors have been successfully applied to the determination of SMX in different water samples. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：48 / 53

页数：6

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