Fluorescence chemosensing of water-soluble Ag14 nanoclusters for lysozyme and Hg2+ ions

被引:22
作者
Ding, Weihua [1 ,2 ]
Guan, Lingmei [1 ]
Han, Juanjuan [1 ]
Mangala, Rajini [1 ]
Luo, Zhixun [1 ,2 ]
机构
[1] Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China
[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
关键词
Ag-14; NCs; NBO analysis; Fluorescence chemosensing; Lysozyme; Hg2+ detection; Bioimaging; SILVER NANOPARTICLE CLUSTERS; FUNCTIONAL THEORY METHOD; GOLD NANOCLUSTERS; STRUCTURAL EVOLUTION; COLORIMETRIC SENSOR; GENETIC ALGORITHM; QUANTUM CLUSTERS; PROTEIN; SPECTROSCOPY; AGGREGATION;
D O I
10.1016/j.snb.2017.04.172
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Employing glutathione (HSG) as a protecting ligand, we have synthesized water-soluble and biocompatible Ag-14(SG)(12) nanoclusters (Ag-14 NCs) of which the composition and structure are determined via high-resolution mass spectrometry, fingerprint spectroscopy and DFT calculations. Natural bond orbital (NBO) analysis on a basis of first-principles theoretical calculations demonstrates the donor-acceptor orbital overlaps and charge-transfer interactions between silver and the ligand HSG. Further, it is interestingly found that the fluorescence of Ag-14 NCs can be largely enhanced at the presence of lysozyme distinguishing from other proteases which we have tested, revealing the feasibility of Ag-14 NCs as chemosensing to identify lysozyme. Also found is that, the fluorescence on-state of the obtained Ag14NCs-Lys can be exclusively switched off at the addition of Hg2+ ions with an ultralow detection limit of similar to 16.2 nM. This interesting finding reveals Ag-14 NCs a versatile chemosensor to identify lysozyme from proteases and to detect mercury ions from various cations. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:364 / 371
页数:8
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