Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae

被引：18

作者：

Foulon, Valentin ^{[1
]}

Artigaud, Sebastien ^{[1
]}

Buscaglia, Manon ^{[1
]}

Bernay, Benoit ^{[2
]}

Fabioux, Caroline ^{[1
]}

Petton, Bruno ^{[3
]}

Elies, Philippe ^{[4
]}

Boukerma, Kada ^{[5
]}

Hellio, Claire ^{[1
]}

Guerard, Fabienne ^{[1
]}

Boudry, Pierre ^{[3
]}

机构：

[1] Inst Univ Europeen Mer, UMR CNRS UBO IRD Ifremer 6539, Lab Sci Environm Marin LEMAR, Technopole Brest Iroise,Rue Dumont dUrville, F-29280 Plouzane, France

[2] Univ Caen Basse Normandie, Plateforme Proteogen SF ICORE, F-14032 Caen, France

[3] IFREMER, UMR CNRS UBO IRD Ifremer 6539, Ctr Bretagne, Lab Sci Environm Marin, F-29280 Plouzane, France

[4] Univ Bretagne Occidentale, Plateforme Imagerie & Mesures Microscopie, F-29200 Brest, France

[5] IFREMER, Ctr Bretagne, LDCM, F-29280 Plouzane, France

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

关键词：

OSTREA-EDULIS; TUBE FEET; IN-SITU; PACIFIC OYSTER; PEDIVELIGER; ADHESION; FOOT; ULTRASTRUCTURE; HISTOCHEMISTRY; METAMORPHOSIS;

D O I：

10.1038/s41598-018-33720-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the adhesive is produced by several foot glands. This adhesive is composed of numerous fibres of differing structure, suggesting differences in chemical composition and function. Fourier transformed infrared spectroscopy indicated a mainly proteinaceous composition. Proteomic analysis of footprints was able to identify 42 proteins, among which, one uncharacterized protein was selected on the basis of its pediveliger transcriptome specificity and then located by mRNA in situ hybridization, revealing its potential role during substrate exploration before oyster larva settlement.

引用

页数：14

共 71 条

[1] Structural and Compositional Characterization of the Adhesive Produced by Reef Building Oysters [J].