Detection of bladder cancer with aberrantly fucosylated ITGA3

被引：16

作者：

Islam, Md Khirul ^{[1
]}

Syed, Parvez ^{[2
]}

Dhondt, Bert ^{[3
,4
,5
]}

Gidwani, Kamlesh ^{[1
]}

Pettersson, Kim ^{[1
]}

Lamminmaki, Urpo ^{[1
]}

Leivo, Janne ^{[1
]}

机构：

[1] Univ Turku, Dept Life Technol, Div Biotechnol, Turku, Finland

[2] Inme Oy, Satakunnantie 8C, Turku 20100, Finland

[3] Ghent Univ Hosp, Dept Urol, Ghent, Belgium

[4] Univ Ghent, Dept Radiat Oncol & Expt Canc Res, Lab Expt Canc Res, Ghent, Belgium

[5] Univ Ghent, Canc Res Inst, Ghent, Belgium

来源：

ANALYTICAL BIOCHEMISTRY | 2021年 / 628卷

基金：

芬兰科学院;

关键词：

Bladder cancer; Integrin; ITGA3; UEA-lectin; Urine; Glycosylations; NANOPARTICLES; URINE; ASSAY; PSA;

D O I：

10.1016/j.ab.2021.114283

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a simple, non-invasive assay to identify fucosylated-glycoisoform of integrin alpha-3 (ITGA3) directly from unprocessed urine. ITGA3 was detected directly from the urine of bladder cancer (BlCa) (n = 13) and benign prostatic hyperplasia (BPH) (n = 9) patients with the use of lectins coated on europium-dopednanoparticles (Eu3+-NPs). Lectin Ulex europaeus agglutinin-I (UEA) showed enhanced binding with BlCaderived ITGA3. The evaluation with individual samples showed that a glycovariant ITGA3-UEA assay could significantly discriminate BlCa from BPH patients (p = 0.007). The detection of aberrantly fucosylated-isoform of ITGA3 from urine can be used to distinguish BlCa from age-matched benign controls in a simple sandwich assay.

引用

页数：5

共 24 条

[1] Structural and mechanical functions of integrins [J].

Anderson L.R. ;

Owens T.W. ;

Naylor M.J. .

Biophysical Reviews, 2014, 6 (2) :203-213

[2] Detection of α1 integrin in urine of patients with immunoglobulin a nephropathy [J].

Bank, Jonathan ;

Ben-David, Aharon ;

Doolman, Ram ;

Sela, Ben-Ami ;

Bank, Ilan .

JOURNAL OF INVESTIGATIVE MEDICINE, 2008, 56 (03) :581-586

[3] Exosomal ITGA3 interferes with non-cancerous prostate cell functions and is increased in urine exosomes of metastatic prostate cancer patients [J].

Bijnsdorp, Irene V. ;

Geldof, Albert A. ;

Lavaei, Mehrdad ;

Piersma, Sander R. ;

van Moorselaar, R. Jeroen A. ;

Jimenez, Connie R. .

JOURNAL OF EXTRACELLULAR VESICLES, 2013, 2 (01)

[4] Adhesion and signaling by B cell-derived exosomes: the role of integrins [J].

Clayton, A ;

Turkes, A ;

Dewitt, S ;

Steadman, R ;

Mason, MD ;

Hallett, MB .

FASEB JOURNAL, 2004, 18 (06) :977-+

[5] Unravelling the proteomic landscape of extracellular vesicles in prostate cancer by density-based fractionation of urine [J].

Dhondt, Bert ;

Geeurickx, Edward ;

Tulkens, Joeri ;

Van Deun, Jan ;

Vergauwen, Glenn ;

Lippens, Lien ;

Miinalainen, Ilkka ;

Rappu, Pekka ;

Heino, Jyrki ;

Ost, Piet ;

Lumen, Nicolaas ;

De Wever, Olivier ;

Hendrix, An .

JOURNAL OF EXTRACELLULAR VESICLES, 2020, 9 (01)

[6] Urinary extracellular vesicle biomarkers in urological cancers: From discovery towards clinical implementation [J].

Dhondt, Bert ;

Van Deun, Jan ;

Vermaerke, Silke ;

de Marco, Ario ;

Lumen, Nicolaas ;

De Wever, Olivier ;

Hendrix, An .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2018, 99 :236-256

[7] THE RECOGNITION OF 3 DIFFERENT EPITOPES FOR THE H-TYPE-2 HUMAN BLOOD-GROUP DETERMINANT BY LECTINS OF ULEX-EUROPAEUS, GALACTIA-TENUIFLORA AND PSOPHOCARPUS-TETRAGONOLOBUS (WINGED BEAN) .15. [J].

DU, MH ;

SPOHR, U ;

LEMIEUX, RU .

GLYCOCONJUGATE JOURNAL, 1994, 11 (05) :443-461

[8] Immuno-based detection of extracellular vesicles in urine as diagnostic marker for prostate cancer [J].

Duijvesz, Diederick ;

Versluis, C. Yin L. ;

van der Fels, Christa A. M. ;

Vredenbregt-van den Berg, Mirella S. ;

Leivo, Janne ;

Peltola, Mari T. ;

Bangma, Chris H. ;

Pettersson, Kim S. I. ;

Jenster, Guido .

INTERNATIONAL JOURNAL OF CANCER, 2015, 137 (12) :2869-2878

[9] A sensitive assay to measure biomarker glycosylation demonstrates increased fucosylation of prostate specific antigen (PSA) in patients with prostate cancer compared with benign prostatic hyperplasia [J].

Dwek, Miriam V. ;

Jenks, Andrew ;

Leathem, Anthony J. C. .

CLINICA CHIMICA ACTA, 2010, 411 (23-24) :1935-1939

[10] A Nanoparticle-Lectin Immunoassay Improves Discrimination of Serum CA125 from Malignant and Benign Sources [J].

Gidwani, Kamlesh ;

Huhtinen, Kaisa ;

Kekki, Henna ;

van Vliet, Sandra ;

Hynninen, Johanna ;

Koivuviita, Niina ;

Perheentupa, Anti ;

Poutanen, Matti ;

Auranen, Annika ;

Grenman, Seija ;

Lamminmaki, Urpo ;

Carpen, Olli ;

van Kooyk, Yvette ;

Pettersson, Kim .

CLINICAL CHEMISTRY, 2016, 62 (10) :1390-1400

← 1 2 3 →