Mutation Associated with Orange Fruit Color Increases Concentrations of β-Carotene in a Sweet Pepper Variety (Capsicum annuum L.)

被引:8
作者
Tomlekova, Nasya [1 ]
Spasova-Apostolova, Velichka [2 ]
Pantchev, Ivelin [3 ]
Sarsu, Fatma [4 ]
机构
[1] Maritsa Vegetable Crops Res Inst, Dept Breeding Variety Maintenance & Intro, 32 Brezovsko Shosse St, Plovdiv 4003, Bulgaria
[2] Tobacco & Tobacco Prod Inst, Dept Breeding & Seed Prod, Markovo 4108, Bulgaria
[3] Sofia Univ, Dept Biochem, 8 Dragan Tzankov, Sofia 1164, Bulgaria
[4] IAEA, Plant Breeding & Genet Sect, Joint FAO IAEA Div Nucl Tech Food & Agr, A-1400 Vienna, Austria
关键词
carotenoids; beta-carotene hydroxylase; sweet pepper; mutagenesis; high performance liquid chromatography; molecular characterization; BIOSYNTHESIS; GENES; HYDROXYLASES; INHERITANCE; MUTANTS; RED;
D O I
10.3390/foods10061225
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Pepper is the second most important vegetable crop in Bulgarian agriculture and has become the subject of extensive breeding programs that frequently employ induced mutagenesis. The success of breeding programs can be enhanced by the efficient and integral application of different biochemical and molecular methods to characterize specific mutant alleles. On the other hand, identifying new cost-effective methods is important under a limited-resources environment. In this paper we compare the levels of five health-related carotenoid compounds of fruits (alpha-carotene, beta-carotene, lutein, beta-cryptoxanthin, zeaxanthin) between a mutant variety Oranzheva kapia (possessing high ss-carotene concentration) and a corresponding initial pepper variety Pazardzhishka kapia 794. Both varieties are intended for fresh consumption. Pepper is a major natural source of beta-carotene. It was observed that fruit at both commercial and botanical maturity from mutant variety had greater alpha-carotene and beta-carotene concentrations to the initial variety (7.49 and 1.94 times higher, respectively) meaning that the mutant was superior in fruit quality to the initial genotype. Two hydroxylase enzymes, converting alpha- and beta-carotene to lutein and zeaxanthin, respectively, are known to exist in pepper and are encoded by two genes on chromosomes 3 and 6-CrtZ(chr03) and CrtZ(chr06). The molecular characterization of the mutant variety through locus-specific Polymerase chain reaction amplification, gene cloning and sequencing as well as expression was performed. Our results suggest that the increased ss-carotene accumulation in the mutant variety Oranzheva kapia results from a biosynthetic pathway breakdown due to deletion of CrtZ(chr03) gene.
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页数:16
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