Isolation and characterization of neural stem cells from the adult human olfactory bulb

被引：177

作者：

Pagano, SF

Impagnatiello, F

Girelli, M

Cova, L

Grioni, E

Onofri, M

Cavallaro, M

Etteri, S

Vitello, F

Giombini, S

Solero, CL

Parati, EA

机构：

[1] Ist Neurol Nazl C Besta, Neuropharmacol Lab, I-20133 Milan, Italy

[2] Schering Plough Res Inst, Milan, Italy

[3] Univ Chieti, Dept Oncol & Neurosci, Chieti, Italy

[4] Natl Neurol Inst C Besta, Div Clin Neurosurg 1, Milan, Italy

[5] Natl Neurol Inst C Besta, Div Clin Neurosurg 2, Milan, Italy

来源：

STEM CELLS | 2000年 / 18卷 / 04期

关键词：

neural stem cell; human adult olfactory bulb; transplantation; Parkinson's disease; multipotent precursor; cell migration;

D O I：

10.1634/stemcells.18-4-295

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

We have recently isolated stem cells deriving from the olfactory bulbs of adult patients undergoing particularly invasive neurosurgery. After improving our experimental conditions, we have now obtained neural stem cells according to clonal analysis. The cells can be expanded, established in continuous cell lines and differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes). Also, after exposition to leukemia inhibitory factor, we are able to improve the number of neurons, an ideal biological source for transplantation in various neurodegenerative disorders.

引用

页码：295 / 300

页数：6

共 23 条

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IMPAGNATIELLO F, 2000, 30 ANN M SOC NEUR NE

[12] POPULATION-DYNAMICS OF ADULT-FORMED GRANULE NEURONS OF THE RAT OLFACTORY-BULB [J].