Neural stem cells express Oct-3/4

被引：12

作者：

Chin, Ji-Hyang ^{[1
]}

Shiwaku, Hiroki ^{[1
]}

Goda, Olga ^{[1
]}

Komuro, Akihiko ^{[1
]}

Okazawa, Hitoshi ^{[1
]}

机构：

[1] Tokyo Med & Dent Univ, Dept Neuropathol, Med Res Inst, Bunkyo Ku, Tokyo 1138510, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 388卷 / 02期

关键词：

Oct-3/4; Pseudogene; Neural stem cell; Somatic stem cell; TRANSCRIPTION FACTOR; MAMMALIAN EMBRYO; OCT4; EXPRESSION; SELF-RENEWAL; POU-DOMAIN; PSEUDOGENES;

D O I：

10.1016/j.bbrc.2009.07.165

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

There remain controversy and disagreement on whether Oct-3/4 is expressed in neural stem cells or not. Although many reports had shown expression of Oct-3/4 in somatic stem and progenitor cells, conditional KO mice of Oct-3/4 in neural stem cells did not show any phenotype. Even pseudogenes are suspected for the "false positive" results. However, we here report that Oct-3/4 but not pseudogenes is actually expressed in neural stem cells. Western blot analysis with multiple Oct-3/4 antibodies also showed protein expression of Oct-3/4. The subnuclear localization of Oct-3/4 is clearly different from that of P19 cells, suggesting that Oct-3/4 might be inactivated by other mechanisms than transcriptional repression. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：247 / 251

页数：5

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