Separation of unmodified polystyrene nanosphere standards by capillary zone electrophoresis

被引：32

作者：

Vanifatova, NG

Spivakov, BY

Mattusch, J

Wennrich, R

机构：

[1] UFZ Helmholtz Ctr Environm Res, Environm Res Ctr, Dept Analyt Chem, D-04318 Leipzig, Germany

[2] Russian Acad Sci, Vernadsky Inst Geochem & Analyt Chem, Moscow 117975, Russia

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 898卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

polystyrene nanosphere;

D O I：

10.1016/S0021-9673(00)00850-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Size separation of five unmodified polystyrene nanosphere standards with diameters between 50 and 600 nm has been achieved in phosphate buffer solutions as carrier electrolyte. The electrophoretic mobility increases with particle diameter. Optical spectra was shown to be different for particles of different size. Effects of injection time, applied voltage, pH, and phosphate concentration in carrier electrolyte on particle separation were studied. Under optimal conditions the peak efficiency ranged from 600 to 10 500 theoretical plate numbers depending on nanosphere diameter was achieved. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：257 / 263

页数：7