Evaluation of 1.0 mm i.d. column performances on ultra high pressure liquid chromatography instrumentation

被引：32
作者：
Lestremau, Francois [1 ]
Wu, Di [1 ]
Szucs, Roman [1 ]
机构：
[1] Pfizer Ltd, Global Res & Dev, Sandwich CT13 9NJ, Kent, England
来源：
JOURNAL OF CHROMATOGRAPHY A | 2010年 / 1217卷 / 30期
关键词：
UHPLC; 1.0 mm column; Gradient space; Peak capacity; KINETIC PLOT METHOD; PEAK-CAPACITY; GRADIENT ELUTION; ID COLUMNS; CONVENTIONAL COLUMNS; POROUS PARTICLES; TEMPERATURE; SEPARATIONS; EFFICIENCY; SYSTEMS;
D O I：
10.1016/j.chroma.2010.05.044
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The present study focuses on the evaluation of 1.0 mm i.d. (internal diameter) columns on a commercial Ultra-High Pressure system. These systems have been developed specifically to operate columns with small volumes, typically 2.1 mm id., by reducing extra-column volume dispersion. The use of columns with smaller id. results in a reduced solvent consumption and required sample volume. The evaluation of the columns was carried out with samples containing neutral and pharmaceutical compounds. In isocratic mode, the extra-column volume produced additional band broadening leading to poor performances compared to equivalent 2.1 mm i.d. columns. By increasing the length of the column, the influence of the extra-column bandpreading could be reduced and 75,000 plates were obtained when four columns were coupled. In gradient mode, the effect of the extra-column contribution on efficiency was limited and about 80% of the performance of the 2.1 mm i.d. columns was obtained. Optimum conditions in gradient mode were further investigated by changing flow rate, gradient time and column length. A different approach of the calculation of peak capacity was also considered for the comparison of the influence of these different parameters. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码：4925 / 4933
页数：9
相关论文
共 32 条
[1]   Choosing sample volume to achieve maximum detection sensitivity and resolution with high-performance liquid chromatography columns of 1.0, 2.1 and 4.6 mm ID [J].
Bakalyar, SR ;
Phipps, C ;
Spruce, B ;
Olsen, K .
JOURNAL OF CHROMATOGRAPHY A, 1997, 762 (1-2) :167-185
[2]   Investigation of the validity of the kinetic plot method to predict the performance of coupled column systems operated at very high pressures under different thermal conditions [J].
Cabooter, Deirdre ;
Lestremau, Francois ;
de Villiers, Andre ;
Broeckhoven, Ken ;
Lynen, Frederic ;
Sandra, Pat ;
Desmet, Gert .
JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (18) :3895-3903
[3]   Kinetic plot method as a tool to design coupled column systems producing 100,000 theoretical plates in the shortest possible time [J].
Cabooter, Deirdre ;
Lestremau, Francois ;
Lynen, Frederic ;
Sandra, Pat ;
Desmet, Gert .
JOURNAL OF CHROMATOGRAPHY A, 2008, 1212 (1-2) :23-34
[4]   Very high pressure HPLC with 1 mm id columns [J].
Colón, LA ;
Cintrón, JM ;
Anspach, JA ;
Fermier, AM ;
Swinney, KA .
ANALYST, 2004, 129 (06) :503-504
[5]   Fused-core particle technology as an alternative to sub-2-αm particles to achieve high separation efficiency with low backpressure [J].
Cunliffe, Jennifer M. ;
Maloney, Todd D. .
JOURNAL OF SEPARATION SCIENCE, 2007, 30 (18) :3104-3109
[6]   Influence of frictional heating on temperature gradients in ultra-high-pressure liquid chromatography on 2.1 mm ID columns [J].
de Villiers, A ;
Lauer, H ;
Szucs, R ;
Goodall, S ;
Sandra, P .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1113 (1-2) :84-91
[7]   Evaluation of ultra performance liquid chromatography -: Part I.: Possibilities and limitations [J].
de Villiers, Andre ;
Lestremau, Francois ;
Szucs, Roman ;
Gelebart, Sylvie ;
David, Frank ;
Sandra, Pat .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1127 (1-2) :60-69
[8]   Geometry-independent plate height representation methods for the direct comparison of the kinetic performance of LC supports with a different size or morphology [J].
Desmet, G ;
Clicq, D ;
Gzil, P .
ANALYTICAL CHEMISTRY, 2005, 77 (13) :4058-4070
[9]  
Dolan JW, 2005, LC GC N AM, V23, P130
[10]   1 mm ID poly(styrene-co-divinylbenzene) monolithic columns for high-peak capacity one- and two-dimensional liquid chromatographic separations of intact proteins [J].
Eeltink, Sebastiaan ;
Dolman, Sebastiaan ;
Detobel, Frederik ;
Desmet, Gert ;
Swart, Remco ;
Ursem, Mario .
JOURNAL OF SEPARATION SCIENCE, 2009, 32 (15-16) :2504-2509
1234