Purification of bacteriocins using size-exclusion chromatography

被引：1

作者：

Rather, Irfan A. ^{[1
]}

Hamad, Alshammari Fanar ^{[1
]}

Bajpai, Vivek K. ^{[1
]}

机构：

[1] Yeungnam Univ, Dept Appl Microbiol & Biotechnol, Gyongsan 712749, South Korea

来源：

BANGLADESH JOURNAL OF PHARMACOLOGY | 2016年 / 11卷 / 02期

关键词：

Bacteriocin; Size-exclusion chromatography;

D O I：

10.3329/bjp.v11i2.25862

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The bacteriocin purification involves following main steps. a) Extraction of cell-free-supernatant of bacteria; b) Ammonium sulfate precipitation; c) Dialysis; d) Diafiltration using PVP and e) Size-exclusion chromatography. However, depending on the nature of work, the compound could be further analyzed by Reverse-phase HPLC, NMR, mass spectrometry and sequencing.

引用

页码：281 / 284

页数：4