Aspirin enhances the osteogenic and anti-inflammatory effects of human mesenchymal stem cells on osteogenic BFP-1 peptide-decorated substrates

被引：16
作者：
Li, Yan [1 ,2 ]
Luo, Zuyuan [2 ]
Xu, Xiao [1 ]
Li, Yongliang [1 ]
Zhang, Siqi [2 ]
Zhou, Ping [1 ]
Sui, Yi [1 ]
Wu, Minjie [3 ]
Luo, En [4 ,5 ]
Wei, Shicheng [1 ,2 ]
机构：
[1] Peking Univ, Cent Lab, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Beijing 100081, Peoples R China
[2] Peking Univ, Lab Biomat & Regenerat Med, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
[3] Peking Univ, Sch & Hosp Stomatol, Dent Ctr 2, Beijing 100081, Peoples R China
[4] Sichuan Univ, State Key Lab Oral Dis, Dept Oral & Maxillofacial Surg, West China Sch, Chengdu 610041, Sichuan, Peoples R China
[5] Sichuan Univ, Hosp Stomatol, Chengdu 610041, Sichuan, Peoples R China
来源：
JOURNAL OF MATERIALS CHEMISTRY B | 2017年 / 5卷 / 34期
关键词：
NF-KAPPA-B; CARBOXYMETHYL CHITOSAN; DIFFERENTIATION; ACTIVATION; BIOCOMPATIBILITY; PERIODONTITIS; EXPRESSION; SURFACES; THERAPY; HEALTHY;
D O I：
10.1039/c7tb01732d
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
Several bone diseases, including arthritis, fracture and osteoporosis, have a pathophysiologically important inflammatory component. Sustained inflammation can result in delayed bone healing. Therefore, to promote bone repair, it is important to inhibit inflammatory bone erosion and suppress pro-inflammatory mediators. In this study, aspirin significantly enhanced immunomodulation and osteogenic differentiation in human mesenchymal stem cells (hMSCs). Additionally, an osteogenic BFP-1 peptide-decorated substrate (PS-PEP) enhanced osteogenic differentiation of aspirin-treated hMSCs compared to a pristine substrate. Alkaline phosphatase assay, quantitative real-time polymerase chain reaction, immunostaining and Alizarin Red S staining revealed that aspirin-treated hMSCs cultured on PS-PEP exhibited enhanced osteogenesis compared with untreated cells. Thus, we report here that the anti-inflammatory and osteogenic effects of aspirin promote the activity and osteogenesis of hMSCs. The combination of aspirin and an osteogenic BFP-1 peptide-decorated substrate suppresses the production of pro-inflammatory mediators and promotes osteogenic differentiation of hMSCs; therefore, this novel strategy has potential for application in cell therapy and bone tissue engineering.
引用
收藏
页码：7153 / 7163
页数：11
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