Comparison of MR imaging against physical sectioning to estimate the volume of human cerebral compartments

被引:118
作者
García-Fiñana, M
Cruz-Orive, LM
Mackay, CE
Pakkenberg, B
Roberts, N
机构
[1] Univ Liverpool, Magnet Resonance & Image Anal Res Ctr, MARIARC, Liverpool L69 3BX, Merseyside, England
[2] Univ Cantabria, Fac Sci, Dept Stat & Computat Math, Santander, Spain
[3] Univ Oxford, Warneford Hosp, Dept Psychiat, Oxford OX3 7JX, England
[4] Bispebjerg Hosp, Res Lab Stereol & Neurosci, DK-2400 Copenhagen, Denmark
关键词
Cavalieri volume estimator; formalin-fixed specimens; gray matter; MRI; physical sectioning; point counting; stereology; systematic sampling; white matter;
D O I
10.1016/S1053-8119(02)00021-6
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The purpose of this study was to compare magnetic resonance imaging (MRI) against physical sectioning techniques to estimate the volume of human cerebral hemisphere compartments (cortex, subcortex, and their union, called "total"). The volume of these compartments was estimated postmortem for six human subjects from MRI virtual sections and from physical sections using the Cavalieri design with point counting. Cursory paired I tests revealed no significant differences between the two methods for any of the three compartments considered, although P = 0.06 for the subcortex. A sharper analysis incorporating recent error prediction formulae revealed a significant discrepancy between the two methods in the estimation of subcortex and total volume for three of the specimens. Yet, none of these analyses is adequate to detect possible biases. The incorporation of an explanatory variable, namely hemisphere weight, and the adoption of a specific gravity p = 1.04 g/cm(3) for the material, enabled us to carry out an allometric analysis for the total compartment which revealed a significant bias of the MRI data. The new error prediction formulae are illustrated by way of example, and their accuracy is checked by a resampling experiment on a data set of 274 MRI sections. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:505 / 516
页数:12
相关论文
共 32 条
  • [1] [Anonymous], J MENT SCI
  • [2] [Anonymous], 1964, Handbook of mathematical functions
  • [3] [Anonymous], 1998, UNBIASED STEREOLOGY
  • [4] Precision of Cavalieri sections and slices with local errors
    Cruz-Orive, LM
    [J]. JOURNAL OF MICROSCOPY-OXFORD, 1999, 193 : 182 - 198
  • [5] ON THE PRECISION OF SYSTEMATIC-SAMPLING - A REVIEW OF MATHERONS TRANSITIVE METHODS
    CRUZORIVE, LM
    [J]. JOURNAL OF MICROSCOPY-OXFORD, 1989, 153 : 315 - 333
  • [6] Accuracy and validity of stereology as a quantitative method for assessment of human temporal lobe volumes acquired by magnetic resonance imaging
    Doherty, CP
    Fitzsimons, M
    Holohan, T
    Mohamed, HB
    Farrell, M
    Meredith, GE
    Staunton, H
    [J]. MAGNETIC RESONANCE IMAGING, 2000, 18 (08) : 1017 - 1025
  • [7] Draper N. R., 1966, APPL REGRESSION ANAL
  • [8] New approximations for the variance in Cavalieri sampling
    García-Fiñana, M
    Cruz-Orive, LM
    [J]. JOURNAL OF MICROSCOPY, 2000, 199 (03) : 224 - 238
  • [9] GARCIAFINANA M, 2000, IMAGE ANAL STEREOL, V19, P71
  • [10] GARCIAFINANA M, 2000, THESIS U CANTABRIA