Saccharomyces cerevisiae Shuttle vectors

被引：43

作者：

Gnugge, Robert ^{[1
,2
,3
,4
,5
]}

Rudolf, Fabian ^{[1
,2
]}

机构：

[1] Swiss Fed Inst Technol, D BSSE, Zurich, Switzerland

[2] Swiss Inst Bioinformat, Zurich, Switzerland

[3] Life Sci Zurich PhD Program Mol & Translat Biomed, Zurich, Switzerland

[4] Competence Ctr Personalized Med, Zurich, Switzerland

[5] Columbia Univ, Med Ctr, Dept Microbiol & Immunol, New York, NY 10032 USA

来源：

YEAST | 2017年 / 34卷 / 05期

关键词：

Saccharomyces cerevisiae; plasmids; shuttle vectors; genomic integration; copy number; plasmid loss; 2-MU-M CIRCLE PLASMID; COCKTAIL DELTA-INTEGRATION; MULTIPLE GENE DISRUPTION; COPY-NUMBER INTEGRATION; YEAST STRUCTURAL GENE; 2 MICRON PLASMID; ESCHERICHIA-COLI; RIBOSOMAL DNA; IN-VIVO; CHROMOSOMAL INTEGRATION;

D O I：

10.1002/yea.3228

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Yeast shuttle vectors are indispensable tools in yeast research. They enable cloning of defined DNA sequences in Escherichia coli and their direct transfer into Saccharomyces cerevisiae cells. There are three types of commonly used yeast shuttle vectors: centromeric plasmids, episomal plasmids and integrating plasmids. In this review, we discuss the different plasmid systems and their characteristic features. We focus on their segregational stability and copy number and indicate how to modify these properties. Copyright (C) 2017 John Wiley & Sons, Ltd.

引用

页码：205 / 221

页数：17

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