Stable adhesion of phospholipid vesicles to modified gold surfaces

被引:31
作者
Sofou, S [1 ]
Thomas, JL [1 ]
机构
[1] Columbia Univ, Dept Chem Engn & Appl Chem, New York, NY 10027 USA
基金
美国国家科学基金会;
关键词
vesicle adhesion; diffusion; acetyl-cysteine; fusion; fluorescence; gold substrate;
D O I
10.1016/S0956-5663(02)00153-7
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Phospholipid vesicles are well-studied biomembrane mimics that are of increasing interest in drug delivery, immunoassays, and sensor chips. In a number of biosensor applications it is desirable to be able to adhere vesicles to a surface in a manner which does not result in their rupture or fusion. Such behavior should, in principle, be achievable by controlling the vesicle-surface and vesicle-vesicle interactions. We have varied vesicle composition and charge (phosphatidylcholine, phosphatidylcholine-phosphatidic acid 18 mol%) and solution ionic strength, to study the adhesion of fluorescent vesicles to glass, gold, and gold modified with chemisorbed acetyl-cysteine. The extent of chemisorption was characterized with angle-resolved X-ray photoelectron spectroscopy (ARXPS), and vesicle integrity and behavior was studied using entrapped and lipophilic fluorescent markers, together and in separate measurements. Vesicle fusion (by energy transfer), adhesion of intact vesicles (with entrapped calcein) and diffusion coefficients (by photobleaching recovery) were monitored using confocal fluorescence microscopy. Acetyl-cysteine modified gold surfaces were shown to be appropriate substrates for adhesion of intact vesicles. Finally, as a 'proof of principle' for fluorescence amplification, release of a self-quenching entrapped reporter dye (calcein) by the detergent Triton X-100 was followed in real time. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:445 / 455
页数:11
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