Fluorescence polarization assays for high throughput screening of G protein-coupled receptors

被引：35

作者：

Banks, P ^{[1
]}

Gosselin, M ^{[1
]}

Prystay, L ^{[1
]}

机构：

[1] Adv Bioconcept Co, Montreal, PQ H4P 2R2, Canada

来源：

JOURNAL OF BIOMOLECULAR SCREENING | 2000年 / 5卷 / 03期

关键词：

D O I：

10.1177/108705710000500308

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence polarization assays in 384-well microtiter plates have been demonstrated, The performance is suitable for high throughput drug screening applications with respect to speed of analysis, displaceable signal, precision, and sensitivity to various reagents. Rank order of potency was maintained relative to [I-125]-ligand filtration assays, and the effects of the highly colored compounds, tartrazine and Chicago Sky Blue, were insignificant on the polarization signal up to a concentration of 1 mu M. These attributes suggest that accurate assessment of drug binding can be obtained.

引用

页码：159 / 167

页数：9

共 6 条

[1]

Kolb A, 1997, HIGH THROUGHPUT SCRE, P345

[2] A time-resolved fluorometric assay for galanin receptors [J].