Production of characteristic volatile markers and their relation to Staphylococcus aureus growth status in pork

被引:18
作者
Hu, K. L. [1 ,4 ]
Yu, X. Q. [2 ,5 ]
Chen, J. [1 ,4 ]
Tang, J. N. [1 ,4 ]
Wang, L. Z. [3 ,6 ]
Li, Y. M. [1 ,4 ]
Tang, C. [1 ,4 ]
机构
[1] Southwest Minzu Univ, Coll Life Sci & Technol, Chengdu, Sichuan, Peoples R China
[2] Sichuan Inst Food & Drug Control, Chengdu, Sichuan, Peoples R China
[3] Thermo Fisher Sci Ltd Corp, Chengdu, Sichuan, Peoples R China
[4] South Sect 4 1th Ring Rd, Chengdu, Sichuan, Peoples R China
[5] 8 Xinwen Rd, Chengdu, Sichuan, Peoples R China
[6] Jinjiang Int Bldg,Linjiang West Rd, Chengdu, Sichuan, Peoples R China
关键词
Staphylococcus aureus; Pork; Bacterial volatile compounds; Growth; Correlation; ORGANIC-COMPOUNDS; ESCHERICHIA-COLI; MEAT SPOILAGE; CONTAMINATION; SWINE;
D O I
10.1016/j.meatsci.2019.107956
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The 3-methyl-butanal and 3-methyl-butanoic acid are known as fingerprint compounds from Staphylococcus aureus. In this study, we investigated production of these two volatile biomarkers and their correlation to S. aureus growth in pork. Both 3-methyl-butanal and 3-methyl-butanoic acid presented high specificity for S. aureus in either media or pork. In sterile minced pork and pork broth, production of volatile biomarkers and the growth of S. aureus were significantly correlated for most single cultures. However, for mixed cultures, only 3-methyl-butanoic acid indicated correlations with growth of S. aureus. Similar trending was also discovered in raw pork, where production of 3-methyl-butanoic acid was significantly correlated with the growth of S. aureus, but not for 3-methyl-butanal. In summary, 3-methyl-butanoic acid was a more stable metabolic marker than 3-methyl-butanal which could be used as an indicator for the presence of S. aureus in pork. This rapid, convenient and cost-effective detection approach could be applied in meat industry to achieve specific detection of S. aureus.
引用
收藏
页数:9
相关论文
共 37 条
[1]   Identification of Spoilage Marker Metabolites in Irish Chicken Breast Muscle Using HPLC, GC-MS Coupled with SPME and Traditional Chemical Techniques [J].
Alexandrakis, Dimitris ;
Brunton, Nigel P. ;
Downey, Gerard ;
Scannell, Amalia G. M. .
FOOD AND BIOPROCESS TECHNOLOGY, 2012, 5 (05) :1917-1923
[2]   Catabolism of leucine to branched-chain fatty acids in Staphylococcus xylosus [J].
Beck, HC ;
Hansen, AM ;
Lauritsen, FR .
JOURNAL OF APPLIED MICROBIOLOGY, 2004, 96 (05) :1185-1193
[3]   Metabolite production and kinetics of branched-chain aldehyde oxidation in Staphylococcus xylosus [J].
Beck, HC ;
Hansen, AM ;
Lauritsen, FR .
ENZYME AND MICROBIAL TECHNOLOGY, 2002, 31 (1-2) :94-101
[4]   Volatile Metabolites of Pathogens: A Systematic Review [J].
Bos, Lieuwe D. J. ;
Sterk, Peter J. ;
Schultz, Marcus J. .
PLOS PATHOGENS, 2013, 9 (05)
[5]   Food Safety in Home Kitchens: A Synthesis of the Literature [J].
Byrd-Bredbenner, Carol ;
Berning, Jacqueline ;
Martin-Biggers, Jennifer ;
Quick, Virginia .
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 2013, 10 (09) :4060-4085
[6]   Bacterial populations and the volatilome associated to meat spoilage [J].
Casaburi, Annalisa ;
Piombino, Paola ;
Nychas, George-John ;
Villani, Francesco ;
Ercolini, Danilo .
FOOD MICROBIOLOGY, 2015, 45 :83-102
[7]   Activities of strains of Brochothrix thermosphacta in vitro and in meat [J].
Casaburi, Annalisa ;
De Filippis, Francesca ;
Villani, Francesco ;
Ercolini, Danilo .
FOOD RESEARCH INTERNATIONAL, 2014, 62 :366-374
[8]   Characteristics of volatile organic compounds produced from five pathogenic bacteria by headspace-solid phase micro-extraction/gas chromatography-mass spectrometry [J].
Chen, Juan ;
Tang, Junni ;
Shi, Hui ;
Tang, Cheng ;
Zhang, Rong .
JOURNAL OF BASIC MICROBIOLOGY, 2017, 57 (03) :228-237
[9]   Growth and Volatile Compounds of Escherichia coli O157:H7 on Irradiated Pork [J].
Chen, Juan ;
Tang, Junni ;
Tang, Cheng ;
Shi, Hui ;
Gu, Huaiyin ;
Lu, Xiaozhu .
JOURNAL OF FOOD QUALITY, 2016, 39 (06) :766-772
[10]   Changes in microbial contamination levels of porcine carcasses and fresh pork in slaughterhouses, processing lines, retail outlets, and local markets by commercial distribution [J].
Choi, Y. M. ;
Park, H. J. ;
Jang, H. I. ;
Kim, S. A. ;
Imm, J. Y. ;
Hwang, I. G. ;
Rhee, M. S. .
RESEARCH IN VETERINARY SCIENCE, 2013, 94 (03) :413-418