Functional complementation by electroporation of human BACs into mammalian fibroblast cells

被引：16

作者：

Hejna, JA ^{[1
]}

Johnstone, PL ^{[1
]}

Kohler, SL ^{[1
]}

Bruun, DA ^{[1
]}

Reifsteck, CA ^{[1
]}

Olson, SB ^{[1
]}

Moses, RE ^{[1
]}

机构：

[1] Oregon Hlth Sci Univ, Dept Mol & Med Genet, Portland, OR 97201 USA

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 04期

关键词：

D O I：

10.1093/nar/26.4.1124

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.

引用

页码：1124 / 1125

页数：2

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