High Spatiotemporal Resolution Imaging with Localized Plasmonic Structured Illumination Microscopy

被引：57
作者：
Bezryadina, Anna [1 ]
Zhao, Junxiang [1 ]
Xia, Yang [2 ]
Zhang, Xiang [2 ]
Liu, Zhaowei [1 ]
机构：
[1] Univ Calif San Diego, Dept Elect & Comp Engn, La Jolla, CA 92093 USA
[2] Univ Calif Berkeley, Dept Mech Engn, Berkeley, CA 94720 USA
来源：
ACS NANO | 2018年 / 12卷 / 08期
基金：
美国国家科学基金会;
关键词：
super-resolution microscopy; plasmonics; structure illumination microscopy; live-cell imaging; nanofabrication; LPSIM; OPTICAL RECONSTRUCTION MICROSCOPY; FLUORESCENCE MICROSCOPY; STIMULATED-EMISSION; LIVE CELLS; LIMIT; LIGHT;
D O I：
10.1021/acsnano.8b03477
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Localized plasmonic structured illumination microscopy (LPSIM) provides multicolor wide-field super resolution imaging with low phototoxicity and high-speed capability. LPSIM utilizes a nanoscale plasmonic antenna array to provide a series of tunable illumination patterns beyond the traditional diffraction limit, allowing for enhanced resolving powers down to a few tens of nanometers. Here, we demonstrate wide-field LPSIM with 50 nm spatial resolution at video rate speed by imaging microtubule dynamics with low illumination power intensity. The design of the LPSIM system makes it suitable for imaging surface effects of cells and tissues with regular sample preparation protocols. LPSIM can be extended to much higher resolution, representing an excellent technology for live-cell imaging of protein dynamics and interactions.
引用
收藏
页码：8248 / 8254
页数：7
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