Droplet electric separator microfluidic device for cell sorting

被引：76
作者：
Guo, Feng
Ji, Xing-Hu
Liu, Kan
He, Rong-Xiang
Zhao, Li-Bo
Guo, Zhi-Xiao
Liu, Wei
Guo, Shi-Shang
Zhao, Xing-Zhong [1 ]
机构：
[1] Wuhan Univ, Minist Educ, Key Lab Artificial Micro & Nanostruct, Wuhan 430072, Peoples R China
来源：
APPLIED PHYSICS LETTERS | 2010年 / 96卷 / 19期
基金：
中国国家自然科学基金;
关键词：
drops; flow separation; microfluidics;
D O I：
10.1063/1.3360812
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
A simple and effective droplet electric separator microfluidic device was developed for cell sorting. The aqueous droplet without precharging operation was influenced to move a distance in the channel along the electric field direction by applying dc voltage on the electrodes beside the channel, which made the target droplet flowing to the collector. Single droplet can be isolated in a sorting rate of similar to 100 Hz with microelectrodes under a required pulse. Single or multiple mammalian cell (HePG2) encapsulated in the surfactant free alginate droplet could be sorted out respectively. This method may be used for single cell operation or analysis.(C) 2010 American Institute of Physics. [doi: 10.1063/1.3360812]
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页数：3
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共 18 条
[11]   Shape-controlled production of biodegradable calcium alginate gel microparticles using a novel microfluidic device [J].
Liu, Kan ;
Ding, Hui-Jiang ;
Liu, Jing ;
Chen, Yong ;
Zhao, Xing-Zhong .
LANGMUIR, 2006, 22 (22) :9453-9457
[12]   Microfluidic devices for the high-throughput chemical analysis of cells [J].
McClain, MA ;
Culbertson, CT ;
Jacobson, SC ;
Allbritton, NL ;
Sims, CE ;
Ramsey, JM .
ANALYTICAL CHEMISTRY, 2003, 75 (21) :5646-5655
[13]   Electrowetting-based actuation of liquid droplets for microfluidic applications [J].
Pollack, MG ;
Fair, RB ;
Shenderov, AD .
APPLIED PHYSICS LETTERS, 2000, 77 (11) :1725-1726
[14]   Non-coalescence of oppositely charged drops [J].
Ristenpart, W. D. ;
Bird, J. C. ;
Belmonte, A. ;
Dollar, F. ;
Stone, H. A. .
NATURE, 2009, 461 (7262) :377-380
[15]   The potential of microfluidic water-in-oil droplets in experimental biology [J].
Schaerli, Yolanda ;
Hollfelder, Florian .
MOLECULAR BIOSYSTEMS, 2009, 5 (12) :1392-1404
[16]   Analysis of single mammalian cells on-chip [J].
Sims, Christopher E. ;
Allbritton, Nancy L. .
LAB ON A CHIP, 2007, 7 (04) :423-440
[17]   Microvalve-actuated precise control of individual droplets in microfluidic devices [J].
Zeng, Shaojiang ;
Li, Bowei ;
Su, Xiao'ou ;
Qin, Jianhua ;
Lin, Bingcheng .
LAB ON A CHIP, 2009, 9 (10) :1340-1343
[18]   Generation of Janus alginate hydrogel particles with magnetic anisotropy for cell encapsulation [J].
Zhao, L. B. ;
Pan, L. ;
Zhang, K. ;
Guo, S. S. ;
Liu, W. ;
Wang, Y. ;
Chen, Y. ;
Zhao, X. Z. ;
Chan, H. L. W. .
LAB ON A CHIP, 2009, 9 (20) :2981-2986
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