Production, purification, and characterization of Streptomyces sp. strain MPPS2 extracellular pyomelanin pigment

被引:15
作者
Bayram, Sinan [1 ]
机构
[1] Bayburt Univ, Vocat Sch Hlth Serv, Dept Med Serv & Tech, TR-69000 Bayburt, Turkey
关键词
Pyomelanin; Bioproduction; Characterization; XRD; FT-IR; H-1; NMR; MELANIN; NANOPARTICLES; NEUROMELANIN; RADIATION; DIVERSITY;
D O I
10.1007/s00203-021-02437-w
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Melanins are natural pigments widely distributed in nature from bacteria to humans. These complex, negatively charged, amorphous, high molecular mass natural biopolymers have many different bioactive properties such as antimicrobial, antiviral, antioxidant, liver protective effects, etc. In this study, some chemical and physical properties of the purified extracellular pyomelanin pigment were investigated via XRD (X-ray diffraction), FT-IR (Fourier transform infrared), and H-1 NMR (Nuclear magnetic resonance). Additionally, the melanin pigment producer Streptomyces sp. strain MPPS2 was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) and 16S rDNA sequence analysis. 16S rDNA nucleotide sequence analysis result was deposited in NCBI GenBank(R) under accession number MT825616.
引用
收藏
页码:4419 / 4426
页数:8
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