Immunogenicity of recombinant LT-B delivered orally to humans in transgenic corn

被引：121

作者：

Tacket, CO

Pasetti, MF

Edelman, R

Howard, JA

Streatfield, S

机构：

[1] Univ Maryland, Sch Med, Ctr Vaccine Dev, Baltimore, MD 21201 USA

[2] ProdiGene, College Stn, TX 77845 USA

来源：

VACCINE | 2004年 / 22卷 / 31-32期

关键词：

transgenic plant; oral vaccine; E; coli;

D O I：

10.1016/j.vaccine.2004.01.073

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Previous clinical studies have demonstrated the feasibility of using edible transgenic plants to deliver protective antigens as new oral vaccines. Transgenic corn is particularly attractive for this purpose since the recombinant antigen is stable and homogeneous, and corn can be formulated in several edible forms without destroying the cloned antigen. Transgenic corn expressing 1 mg of LT-B of Escherichia coli without buffer was fed to adult volunteers in three doses, each consisting of 2.1 g of plant material. Seven (78%) of nine volunteers developed rises in both serum IgG anti-LT and numbers of specific antibody secreting cells after vaccination. Four (44%) of nine volunteers also developed stool IgA. Transgenic plants represent a new vector for oral vaccine antigens. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：4385 / 4389

页数：5

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