Analysis of the ligand-binding domain of macrophage colony-stimulating receptor

被引：3
|
作者：
Luo, SQ
Zheng, DX [1 ]
Liu, YX
Rao, Q
Wu, KF
机构：
[1] Peking Union Med Coll, Chinese Acad Med Sci, Inst Hematol, State Key Lab Expt Hematol, Tianjin 300020, Peoples R China
[2] Peking Union Med Coll, Chinese Acad Med Sci, Inst Basic Med Sci, State Key Lab Med Mol Biol, Beijing 100005, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2000年 / 45卷 / 13期
关键词：
M-CSF; binding ability; structure-function;
D O I：
10.1007/BF02886077
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
RNAs have been extracted from human placenta. Extracellular regions of M-CSFR, D1-3, D2-3 and D3 motifs have been amplified with RT-PCR. The proteins have been expressed in E.coli. Enzyme-linked immusorbent assay (EIA) shows that recombinant D1-3 possesses binding ability 3 times that of D2-3. Kd, the dissociation constant of the former is 11 nmol/L, and that of latter is 39 nmol/L. D3 lacks binding ability. These data suggest that D2-3 is the main site for M-CSF binding, D1 is an assistant site and also contributes to the conformation of site for ligand binding.
引用
收藏
页码：1191 / 1195
页数：5
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