Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix

被引：20

作者：

Shao, Yi ^{[1
,2
,4
]}

Quyang, Luowa ^{[1
]}

Zhou, Yueping ^{[1
]}

Tang, Jing ^{[1
,3
]}

Tan, Yuehui ^{[1
]}

Liu, Qiuping ^{[1
]}

Lin, Zhirong ^{[1
,4
]}

Yin, Tingting ^{[1
]}

Qiu, Fangfang ^{[1
]}

Liu, Zuguo ^{[1
]}

机构：

[1] Xiamen Univ, Inst Eye, Fujian Prov Key Lab Ophthalmol & Vis Res, Xiamen 361005, Fujian, Peoples R China

[2] Nanchang Univ, Affiliated Hosp 1, Nanchang, Jiangxi, Peoples R China

[3] Xiamen Univ, Affiliated Zhongshan Hosp, Xiamen 361005, Fujian, Peoples R China

[4] Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510275, Guangdong, Peoples R China

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL | 2010年 / 46卷 / 07期

关键词：

Cornea matrix; Human sera; Tissue engineering; Decellularization biocompatibily; CROSS-LINKED COLLAGEN; XENOTRANSPLANTATION; EQUIVALENT; SCAFFOLD; PROGRESS; BABOONS; STROMA; RABBIT;

D O I：

10.1007/s11626-010-9328-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This study was to investigate the stability, physico-mechanical property and biocompatibility of porcine corneal acellularized matrix (PCAM) that was prepared using human sera treatment to decellularize corneas. The stability (the rate of biodegradation) and physico-mechanical property (water uptake, density, and porosity) of PCAM were not compromised, compared with porcine fresh cornea matrix (PFCM, p > 0.05). The contact and extract cytotoxicity tests with human corneal epithelial cells and human keratocytes showed that PCAM has a good biocompatibility ex vivo and no cytotoxic effect. These results present the ability to create safety scaffolds that function as cornea grafts and provide a novel experimental approach for the study of cornea tissue engineering using acellular porcine cornea.

引用

页码：600 / 605

页数：6

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