Experimental Evaluation of Long-Term Toxic Effects of Methotrexate on Male Reproductive System

被引：2

作者：

Borovskaya, T. G. ^{[1
]}

Shchemerova, Yu A. ^{[1
]}

Bokhan, E. A. ^{[1
]}

Grigor'eva, V. A. ^{[1
]}

Vychuzhanina, A. V. ^{[1
]}

Poluektova, M. E. ^{[1
]}

Goldberg, V. E. ^{[2
]}

Dygai, A. M. ^{[1
]}

机构：

[1] Tomsk Natl Res Med Ctr, ED Goldberg Res Inst Pharmacol & Regenerat Med, Tomsk, Russia

[2] Tomsk Natl Res Med Ctr, Canc Res Inst, Tomsk, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2021年 / 171卷 / 01期

关键词：

methotrexate; delayed toxic effects; rats; mice; spermatogenesis; CELLS;

D O I：

10.1007/s10517-021-05167-7

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The morphological and functional state of the reproductive system was studied in male outbred rats (SD stock) and male F1(CBAxC57BL/6) mice after long-term (3 months) methotrexate administration. The drug was administered subcutaneously once a week for 4 weeks, the dose for male rats was 1 mg/kg, for male mice 2.2 mg/kg. It was found that male rats retained the ability to conceive, their reproductive potential was not limited by increased risk of embryo death. At the same time, signs of astheno- and pathospermia were revealed. The testicular tissue was characterized by reduced content of the sources of the proliferative pool of spermatogenesis. In mice treated with methotrexate, increased content of DNA breaks was detected in the testicular cells.

引用

页码：37 / 40

页数：4

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