Neuronal plasticity after spinal cord injury: identification of a gene cluster driving neurite outgrowth

被引:69
作者
Di Giovanni, S
Faden, AI
Yakovlev, A
Duke-Cohan, JS
Finn, T
Thouin, M
Knoblach, S
De Biase, A
Bregman, BS
Hoffman, EP
机构
[1] Georgetown Univ, Sch Med, Dept Neurosci, Washington, DC 20057 USA
[2] Childrens Natl Med Ctr, Ctr Genet Med, Washington, DC 20010 USA
[3] Dana Farber Canc Inst D1526, Dept Med Oncol, Boston, MA 02115 USA
[4] Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA
关键词
SCI; microtubule-associated proteins; GAP-43;
D O I
10.1096/fj.04-2694fje
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Functional recovery after spinal cord injury (SCI) may result in part from axon outgrowth and related plasticity through coordinated changes at the molecular level. We employed microarray analysis to identify a subset of genes the expression patterns of which were temporally coregulated and correlated to functional recovery after SCI. Steady-state mRNA levels of this synchronously regulated gene cluster were depressed in both ventral and dorsal horn neurons within 24 h after injury, followed by strong re-induction during the following 2 wk, which paralleled functional recovery. The identified cluster includes neuritin, attractin, microtubule-associated protein 1a, and myelin oligodendrocyte protein genes. Transcriptional and protein regulation of this novel gene cluster was also evaluated in spinal cord tissue and in single neurons and was shown to play a role in axonal plasticity. Finally, in vitro transfection experiments in primary dorsal root ganglion cells showed that cluster members act synergistically to drive neurite outgrowth.
引用
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页码:153 / +
页数:29
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