Gene expression spectra in human leukemia HL-60 cells treated with EGCG

被引：2

作者：

Cao, J ^{[1
]}

Ren, LL

Liu, JW

Li, Y

Qu, HY

机构：

[1] Cent S Univ, Tea & Hlth Lab, Xiangya Sch Med, Changsha 410078, Hunan, Peoples R China

[2] Integrate Tradit Chinese & Western Med Hosp, Beijing, Peoples R China

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2004年 / 556卷 / 1-2期

关键词：

EGCG; HL-60; cells; apoptosis; cDNA chip; RT-PCR; gene differential expression; tea polyphenol;

D O I：

10.1016/j.mrfmmm.2004.08.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To decipher the molecular mechanism of EGCG induced HL-60 cell apoptosis, alterations of gene expression spectra in HL-60 cell line cells after treatment with EGCG were screened by cDNA chip, and analyzed with the GenePix 3.0 twice; and the cDNA chip results further identified by RT-PCR. Ninety-seven genes among the total 8398 (1.15%) showed consistent significant differential expression in the duplicated cDNA chip assessments. Thirty-nine genes (40.2%) were up-regulated and 58 genes (59.8%) were down-regulated; and the randomly selected four performed RT-PCR results agreed with the cDNA chip data. The results suggest that the apoptosis of HL-60 cells induced by EGCG is a progressive transformation process regulated by a variety of genes. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：193 / 200

页数：8

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