Monolithic columns for high-efficiency HPLC separations

被引:103
作者
Ikegami, T [1 ]
Tanaka, N [1 ]
机构
[1] Kyoto Inst Technol, Dept Polymer Sci & Engn, Sakyo Ku, Kyoto 6068585, Japan
关键词
D O I
10.1016/j.cbpa.2004.08.009
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Monolithic columns have been studied as materials, having advantages inherent to their network-type one-piece structures. These columns demonstrably provide generally higher performance than conventional particle-packed columns in pressure-driven liquid chromatography, based on their higher external porosity. The use of such columns has just started in many areas.
引用
收藏
页码:527 / 533
页数:7
相关论文
共 54 条
[1]   Determination of the porosities of monolithic columns by inverse size-exclusion chromatography [J].
Al-Bokari, M ;
Cherrak, D ;
Guiochon, G .
JOURNAL OF CHROMATOGRAPHY A, 2002, 975 (02) :275-284
[2]   Capillary electrochromatography with monolithic silica columns III. Preparation of hydrophilic silica monoliths having surface-bound cyano groups: chromatographic characterization and application to the separation of carbohydrates, nucleosides, nucleic acid bases and other neutral polar species [J].
Allen, D ;
El Rassi, Z .
JOURNAL OF CHROMATOGRAPHY A, 2004, 1029 (1-2) :239-247
[3]   Capillary electrochromatography with monolithic-silica columns. II. Preparation of amphiphilic silica monoliths having surface-bound cationic octadecyl moieties and their chromatographic characterization and application to the separation of proteins and other neutral and charged species [J].
Allen, D ;
El Rassi, Z .
ANALYST, 2003, 128 (10) :1249-1256
[4]   Applications of silica-based monolithic HPLC columns [J].
Cabrera, K .
JOURNAL OF SEPARATION SCIENCE, 2004, 27 (10-11) :843-852
[5]  
CHAKVETADZE B, 2003, CHEM LETT, V32, P850
[6]   High-performance liquid chromatographic enantioseparations on capillary columns containing monolithic [J].
Chankvetadze, B ;
Yamamoto, C ;
Tanaka, N ;
Nakanishi, K ;
Okamoto, Y .
JOURNAL OF SEPARATION SCIENCE, 2004, 27 (10-11) :905-911
[7]   Chemically modified chiral monolithic silica column prepared by a sol-gel process for enantiomeric separation by micro high-performance liquid chromatography [J].
Chen, ZL ;
Uchiyama, K ;
Hobo, T .
JOURNAL OF CHROMATOGRAPHY A, 2002, 942 (1-2) :83-91
[8]   Methacrylate monolithic columns of 320 μm ID for capillary liquid chromatography [J].
Coufal, P ;
Cihák, M ;
Suchánková, J ;
Tesarová, E ;
Bosáková, Z ;
Stulík, K .
JOURNAL OF CHROMATOGRAPHY A, 2002, 946 (1-2) :99-106
[9]   Capillary electrochromatography - A high-efficiency micro-separation technique [J].
Dittmann, MM ;
Rozing, GP .
JOURNAL OF CHROMATOGRAPHY A, 1996, 744 (1-2) :63-74
[10]   Capillary columns with in situ formed porous monolithic packing for micro high-performance liquid chromatography and capillary electrochromatography [J].
Gusev, I ;
Huang, X ;
Horváth, C .
JOURNAL OF CHROMATOGRAPHY A, 1999, 855 (01) :273-290