Induced activity of adenine phosphoribosyltransferase (APRT) in iron-deficient barley roots: a possible role for phytosiderophore production

被引:39
作者
Itai, R
Suzuki, K
Yamaguchi, H
Nakanishi, H
Nishizawa, NK
Yoshimura, E
Mori, S
机构
[1] Univ Tokyo, Dept Appl Biol Chem, Lab Plant Mol Physiol, Bunkyo Ku, Tokyo 1138657, Japan
[2] Japan Sci & Technol Corp, CREST, Tsukuba, Ibaraki 3050047, Japan
关键词
APRT; barley; Fe deficiency; methionine cycle; mugineic acid;
D O I
10.1093/jexbot/51.348.1179
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
To isolate the genes involved in the response of graminaceous plants to Fe-deficient stress, a protein induced by Fe-deficiency treatment was isolated from barley (Hordeum vulgare L.) roots. Based on the partial amino acid sequence of this protein, a cDNA (HvAPT1) encoding adenine phosphoribosyltransferase (APRT: EC 2.4.2.7) was cloned from a cDNA library prepared from Fe-deficient barley roots, Southern analysis suggested that there were at least two genes encoding APRT in barley, Fe deficiency increased HvAPT1 expression in barley roots and resupplying Fe to the Fe-deficient plants rapidly negated the increase in HvAPT1 mRNA. Analysis of localization of HvAPT1-sGFP fusion proteins in tobacco BY-2 cells indicated that the protein from HvAPT1 was localized in the cytoplasm of cells. Consistent with the results of Northern analysis, the enzymatic activity of APRT in barley roots was remarkably increased by Fe deficiency. This induction of APRT activity by Fe deficiency was also observed in roots of other graminaceous plants such as rye, maize, and rice. In contrast, the induction was not observed to occur in the roots of a non-graminaceous plant, tobacco. Graminaceous plants generally synthesize the mugineic acid family phytosiderophores (MAs) in roots under Fe-deficient conditions. In this paper, a possible role of HvAPT1 in the biosynthesis of MAs related to adenine salvage in the methionine cycle is discussed.
引用
收藏
页码:1179 / 1188
页数:10
相关论文
共 36 条
  • [1] PURINE METABOLISM AND ITS REGULATION IN HIGHER-PLANTS .3. PRESENCE OF ADENINE PHOSPHORIBOSYLTRANSFERASE AND ADENOSINE KINASE IN CHLOROPLASTS OF SPINACH LEAVES
    ASHIHARA, H
    UKAJI, T
    [J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1985, 17 (11): : 1275 - 1277
  • [2] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [3] METABOLISM OF PURINE NUCLEOTIDES IN THE TOMATO PLANT
    BURCH, LR
    STUCHBURY, T
    [J]. PHYTOCHEMISTRY, 1986, 25 (11) : 2445 - 2449
  • [4] Engineered GFP as a vital reporter in plants
    Chiu, WL
    Niwa, Y
    Zeng, W
    Hirano, T
    Kobayashi, H
    Sheen, J
    [J]. CURRENT BIOLOGY, 1996, 6 (03) : 325 - 330
  • [5] GENOMIC SEQUENCING
    CHURCH, GM
    GILBERT, W
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07): : 1991 - 1995
  • [6] DEBOER JG, 1991, J MOL BIOL, V221, P163, DOI 10.1016/0022-2836(91)80212-D
  • [7] RAPID PRODUCTION OF FULL-LENGTH CDNAS FROM RARE TRANSCRIPTS - AMPLIFICATION USING A SINGLE GENE-SPECIFIC OLIGONUCLEOTIDE PRIMER
    FROHMAN, MA
    DUSH, MK
    MARTIN, GR
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) : 8998 - 9002
  • [8] INVIVO METABOLISM OF 5'-METHYLTHIOADENOSINE IN LEMNA
    GIOVANELLI, J
    DATKO, AH
    MUDD, SH
    THOMPSON, GA
    [J]. PLANT PHYSIOLOGY, 1983, 71 (02) : 319 - 326
  • [9] Cloning of nicotianamine synthase genes, novel genes involved in the biosynthesis of phytosiderophores
    Higuchi, K
    Suzuki, K
    Nakanishi, H
    Yamaguchi, H
    Nishizawa, NK
    Mori, S
    [J]. PLANT PHYSIOLOGY, 1999, 119 (02) : 471 - 479
  • [10] LEE D, 1993, PHYSIOL PLANTARUM, V87, P483, DOI 10.1111/j.1399-3054.1993.tb02497.x