Polymerase chain reaction ribotypes of Clostridium difficile in hospitals in England and Wales

被引：0

作者：

Brazier, JS ^{[1
]}

O'Neill, GL ^{[1
]}

Duerden, BI ^{[1
]}

机构：

[1] Univ Wales Hosp, Publ Hlth Lab, Anaerobe Reference Unit, Cardiff CF4 4XW, S Glam, Wales

来源：

REVIEWS IN MEDICAL MICROBIOLOGY | 1997年 / 8卷

关键词：

Clostridium difficile; PCR ribotyping; hospital infection;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A polymerase chain reaction (PCR) ribotyping scheme was applied to strains of Clostridium difficile from 53 hospitals throughout England and Wales. PCR ribotype type 1 was by far the most common genotype and was present in 30/53 (57%) of the hospitals investigated. PCR products from 40 type 1 strains from 22 hospitals were digested using 11 different restriction enzymes. This analysis revealed no differences in the 16S-23S intergenic spacer region of these strains indicating a high level of homogeneity within this PCR ribotype.

引用

页码：S55 / S56

页数：2

共 4 条

[1] TYPING OF CLOSTRIDIUM-DIFFICILE STRAINS BY PCR-AMPLIFICATION OF VARIABLE-LENGTH 16S-23S RDNA SPACER REGIONS
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ONeill, GL
Ogunsola, FT
Brazier, JS
Duerden, BI
[J]. ANAEROBE, 1996, 2 (04) : 205 - 209
[4] A comparison of PCR ribotyping and pyrolysis mass spectrometry for typing clinical isolates of Clostridium difficile
ONeill, GL
Brazier, JS
Magee, JT
Duerden, BI
[J]. ANAEROBE, 1996, 2 (04) : 211 - 215

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