Modulation of the N-type calcium channel gene expression by the α subunit of Go

被引:4
作者
Kim, BJ
Ghil, SH
Kim, MJ
Park, SY
Kim, DS
Kim, SH
Chin, H
Birnbaumer, L
Jiang, M
Hong, SY
Suh-Kim, H
Lee, YD
机构
[1] Ajou Univ, Sch Med, Dept Anat, Paldal Gu, Suwon 442749, South Korea
[2] Ajou Univ, Dept Pediat, Suwon 441749, South Korea
[3] Ajou Univ, Brain Dis Res Ctr, Suwon 441749, South Korea
[4] Kyungpook Natl Univ, Dept Anat, Taegu 702701, South Korea
[5] NIMH, Genet Res Branch, DNBBS, NIH, Bethesda, MD USA
[6] Univ Calif Los Angeles, Dept Anesthesiol, Los Angeles, CA 90024 USA
[7] NIEHS, Lab Signal Transduct, Res Triangle Pk, NC 27709 USA
[8] Sungkyunkwan Univ, Dept Gen Engn, Suwon 440746, South Korea
来源
MOLECULAR BRAIN RESEARCH | 2003年 / 112卷 / 1-2期
基金
新加坡国家研究基金会;
关键词
N-type calcium channel; Go; cAMP; F11; neuron; differentiation;
D O I
10.1016/S0169-328X(03)00053-6
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Go, a heterotrimeric G-protein, is enriched in brain and neuronal growth cones. Although several reports suggest that Go may be involved in modulation of neuronal differentiation, the precise role of Go is not clear. To investigate the function of Go in neuronal differentiation, we determined the effect of Goalpha, the a subunit of Go, on the expression of Ca(v)2.2, the pore-forming unit of N-type calcium channels, at the transcription level. Treatment with cyclic AMP (cAMP), which triggers neurite outgrowth in neuroblastoma F11 cells, increased the mRNA level and the promoter activity of the Ca(v)2.2 gene. Overexpression of Goa inhibited neurite extension in F11 cells and simultaneously repressed the stimulatory effect of cAMP on the Ca(v)2.2 gene expression to the basal level. Targeted mutation of the Goa gene also increased the level of Ca(v)2.2 in the brain. These results suggest that Go may regulate neuronal differentiation through modulation of gene expression of target genes such as N-type calcium channels. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:95 / 102
页数:8
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