Modulation of endothelial cell expression of ICAM-1, E-selectin, and VCAM-1 by beta-estradiol, progesterone, and dexamethasone

被引:94
作者
Aziz, KE
Wakefield, D
机构
[1] UNIV NEW S WALES, PRINCE HENRY HOSP, IMMUNOPATHOL DEPT, SCH PATHOL, LITTLE BAY, NSW 2036, AUSTRALIA
[2] UNIV NEW S WALES, PRINCE WALES HOSP, SCH PATHOL, LITTLE BAY, NSW 2036, AUSTRALIA
关键词
D O I
10.1006/cimm.1996.0010
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The aim of this study was to examine the effects of beta-estradiol, progesterone, and dexamethasone on cytokine-stimulated endothelial cell expression of adhesion molecules. TNF-alpha (250 U/ml) and IL-l alpha (50 U/ml) were used to stimulate the endothelial cells for 6 or 23 hr in vitro. Indirect immunofluorescence and flow cytometry were used to quantitate expression of adhesion molecules, After 6 hr stimulation with TNF-alpha increased expression of E-selectin (P < 0.03) was noted with beta-estradiol, Strong suppression of ICAM-1 (P < 0.005) and E-selectin (P < 0.005) expression was evident with dexamethasone, which did not influence VCAM-1 expression, After 6 hr stimulation with IL-l alpha suppression of E-selectin was observed with progesterone (P < 0.001). Dexamethasone had strong suppressive effects on ICAM-1 (P < 0.001), E-selectin (P < 0.0001), and VCAM-1 (P < 0.0002), After 23 hr stimulation with IL-1 alpha or TNF-alpha none of the examined steroids showed a significant effect on the fluorescence intensity of adhesion molecules, although there was a slight increase of the percentage of ICAM-1-positive cells with high concentrations of beta-estradiol after stimulation with TNF-alpha. beta-Estradiol and progesterone are modulatory factors of E-selectin expression on endothelial cell in vitro. Dexamethasone reduces adhesion molecule expression over endothelial cells after cytokine stimulation. These effects may be important in understanding the role of these steroids in autoimmune diseases. (C) 1996 Academic Press, Inc.
引用
收藏
页码:79 / 85
页数:7
相关论文
共 34 条
  • [1] AHMED SA, 1989, J IMMUNOL, V142, P2647
  • [2] AHMED SA, 1985, AM J PATHOL, V121, P531
  • [3] SEX HORMONAL EFFECTS ON THE SEVERITY OF STREPTOCOCCAL CELL-WALL INDUCED POLYARTHRITIS IN THE RAT
    ALLEN, JB
    BLATTER, D
    CALANDRA, GB
    WILDER, RL
    [J]. ARTHRITIS AND RHEUMATISM, 1983, 26 (04): : 560 - 563
  • [4] Aziz K E, 1992, J Clin Lab Immunol, V37, P39
  • [5] SJOGRENS-SYNDROME - REVIEW WITH RECENT INSIGHTS INTO IMMUNOPATHOGENESIS
    AZIZ, KE
    MONTANARO, A
    MCCLUSKEY, PJ
    WAKEFIELD, D
    [J]. AUSTRALIAN AND NEW ZEALAND JOURNAL OF MEDICINE, 1992, 22 (06): : 671 - 678
  • [6] GENE-REGULATION BY STEROID-HORMONES
    BEATO, M
    [J]. CELL, 1989, 56 (03) : 335 - 344
  • [7] IDENTIFICATION OF AN INDUCIBLE ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE
    BEVILACQUA, MP
    POBER, JS
    MENDRICK, DL
    COTRAN, RS
    GIMBRONE, MA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (24) : 9238 - 9242
  • [8] CAMPISI D, 1987, INT J TISSUE REACT, V9, P393
  • [9] CARTER BZ, 1991, LAB INVEST, V65, P610
  • [10] ESTRADIOL ENHANCES LEUKOCYTE BINDING TO TUMOR-NECROSIS-FACTOR (TNF)-STIMULATED ENDOTHELIAL-CELLS VIA AN INCREASE IN TNF-INDUCED ADHESION MOLECULES E-SELECTIN, INTERCELLULAR-ADHESION MOLECULE TYPE-1, AND VASCULAR CELL-ADHESION MOLECULE TYPE-1
    CID, MC
    KLEINMAN, HK
    GRANT, DS
    SCHNAPER, HW
    FAUCI, AS
    HOFFMAN, GS
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1994, 93 (01) : 17 - 25