Using Information and Communication Technologies in teaching and learning activities allows the improvement of the learning process and motivates students, once the information can be presented with online and offline multiple interactive media, involving sounds, narration, images, videos, 3D objects, animations, etc. The study of histology is one subject that can be benefited with the use of multiple media due to the richness of pictorial and graphics information. Microscope images, evaluation graphics, videos and animations are usual in the study of histology. Augmented reality is one of those technologies that promises major changes in Education. Augmented reality applications can be used intuitively, explore many senses and have appeal to motivate students to the study. Thus, this paper presents an experience in developing and using online augmented reality applications related to histology. Those applications are based on the development of Open Educational Resources and their integration through an augmented reality authoring tool suitable for nonspecialists users, such as teachers. The applications were developed with augmented reality exploring the subject skin and skin appendages, which were presented to 15 students of Histology of a medical school. After submitting the application, the students answered a questionnaire giving parameters to analyze some characteristics of the applications, such as user interaction, interface, visual appearance and sound, and innovative features, such as immersion, presence in the application of video and image 3D object on the skin, and an area of activity that allows students to fastening exercising content exposed. The results are discussed in this paper and show a large potential contribution for histology teaching, since most aspects proposed and analyzed demonstrates good acceptance by students, especially regarding to the ease of access and handling visuals and sound resources, including immersion and especially the use of the authoring tool.