Two-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo

被引：22

作者：

Kaszas, Attila ^{[1
,2
,3
]}

Szalay, Gergely ^{[4
]}

Slezia, Andrea ^{[1
,5
]}

Bojdan, Alexandra ^{[4
]}

Vanzetta, Ivo ^{[2
,3
]}

Hangya, Balazs ^{[5
]}

Rozsa, Balazs ^{[4
,6
]}

O'Connor, Rodney ^{[1
,2
,3
]}

Moreau, David ^{[1
]}

机构：

[1] Mines St Etienne, Ctr CMP, Dept BEL, F-13541 Gardanne, France

[2] CNRS, Inst Neurosci Timone, UMR 7289, F-13005 Marseille, France

[3] Aix Marseille Univ, F-13005 Marseille, France

[4] Inst Expt Med, Lab 3D Funct Network & Dendrit Imaging, H-1083 Budapest, Hungary

[5] Inst Expt Med, Lendulet Lab Syst Neurosci, H-1083 Budapest, Hungary

[6] Pazmany Peter Catholic Univ, Fac Informat Technol, Two Photon Lab, H-1083 Budapest, Hungary

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

基金：

欧盟地平线“2020”; 欧洲研究理事会;

关键词：

OPTICAL STIMULATION; LIGHT; CELLS; TEMPERATURE; CORTEX; DAMAGE;

D O I：

10.1038/s41598-021-89163-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Infrared neural stimulation is a promising tool for stimulating the brain because it can be used to excite with high spatial precision without the need of delivering or inserting any exogenous agent into the tissue. Very few studies have explored its use in the brain, as most investigations have focused on sensory or motor nerve stimulation. Using intravital calcium imaging with the genetically encoded calcium indicator GCaMP6f, here we show that the application of infrared neural stimulation induces intracellular calcium signals in Layer 2/3 neurons in mouse cortex in vivo. The number of neurons exhibiting infrared-induced calcium response as well as the amplitude of those signals are shown to be both increasing with the energy density applied. By studying as well the spatial extent of the stimulation, we show that reproducibility of the stimulation is achieved mainly in the central part of the infrared beam path. Stimulating in vivo at such a degree of precision and without any exogenous chromophores enables multiple applications, from mapping the brain's connectome to applications in systems neuroscience and the development of new therapeutic tools for investigating the pathological brain.

引用

页数：18