Binding and molecular basis of the bat coronavirus RaTG13 virus to ACE2 in humans and other species

被引：113

作者：

Liu, Kefang ^{[1
]}

Pan, Xiaoqian ^{[1
,2
]}

Li, Linjie ^{[1
,2
]}

Yu, Feng ^{[3
]}

Zheng, Anqi ^{[1
]}

Du, Pei ^{[1
]}

Han, Pengcheng ^{[1
,4
]}

Meng, Yumin ^{[1
]}

Zhang, Yanfang ^{[1
,5
]}

Wu, Lili ^{[1
]}

Chen, Qian ^{[1
,6
]}

Song, Chunli ^{[6
]}

Jia, Yunfei ^{[1
,7
]}

Niu, Sheng ^{[1
,7
]}

Lu, Dan ^{[1
]}

Qiao, Chengpeng ^{[1
]}

Chen, Zhihai ^{[8
]}

Ma, Dongli ^{[9
]}

Ma, Xiaopeng ^{[9
]}

Tan, Shuguang ^{[1
]}

Zhao, Xin ^{[1
]}

Qi, Jianxun ^{[1
,2
]}

Gao, George F. ^{[1
,2
]}

Wang, Qihui ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China

[4] Emory Univ, Dept Biomed Engn, Atlanta, GA 10033 USA

[5] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Lab Prot Engn & Vaccines, Tianjin 300308, Peoples R China

[6] Anhui Univ, Inst Phys Sci & Informat, Hefei 230039, Peoples R China

[7] Shanxi Agr Univ, Coll Vet Med, Jinzhong 030801, Peoples R China

[8] Capital Med Univ, Beijing Ditan Hosp, Ctr Infect Dis, Beijing 100015, Peoples R China

[9] Shenzhen Childrens Hosp, Shenzhen 518036, Peoples R China

来源：

CELL | 2021年 / 184卷 / 13期

基金：

中国国家自然科学基金;

关键词：

SARS-COV-2; SPIKE; OUTBREAK; COVID-19; DOMAIN; ENTRY; MERS;

D O I：

10.1016/j.cell.2021.05.031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been spreading worldwide, causing a global pandemic. Bat-origin RaTG13 is currently the most phylogenetically related virus. Here we obtained the complex structure of the RaTG13 receptor binding domain (RBD) with human ACE2 (hACE2) and evaluated binding of RaTG13 RBD to 24 additional ACE2 orthologs. By substituting residues in the RaTG13 RBD with their counterparts in the SARS-CoV-2 RBD, we found that residue 501, the major position found in variants of concern (VOCs) 501Y.V1/V2/V3, plays a key role in determining the potential host range of RaTG13. We also found that SARS-CoV-2 could induce strong cross-reactive antibodies to RaTG13 and identified a SARS-CoV-2 monoclonal antibody (mAb), CB6, that could cross-neutralize RaTG13 pseudovirus. These results elucidate the receptor binding and host adaption mechanisms of RaTG13 and emphasize the importance of continuous surveillance of coronaviruses (CoVs) carried by animal reservoirs to prevent another spillover of CoVs.

引用

页码：3438 / +

页数：24

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