Antimicrobial Activity of a Halocidin-Derived Peptide Resistant to Attacks by Proteases

被引:27
作者
Shin, Yong Pyo [1 ]
Park, Ho Jin [1 ]
Shin, Seo Hwa [1 ]
Lee, Young Shin [1 ]
Park, Seungmi [2 ]
Jo, Sungho [3 ]
Lee, Yong Ho [4 ]
Lee, In Hee [1 ,5 ]
机构
[1] Hoseo Univ, Dept Biotechnol, Asan 336795, Chungnam, South Korea
[2] Hoseo Univ, Dept Nursing, Asan 336795, Chungnam, South Korea
[3] Dankook Univ, Coll Med, Dept Surg, Cheonan, South Korea
[4] Catholic Univ Daegu, Dept Med Life Sci, Coll Nat Sci, Gyeongbuk, South Korea
[5] Hoseo Univ, Res Inst Basic Sci, Asan 336795, Chungnam, South Korea
关键词
HOST-DEFENSE PEPTIDES; STRUCTURE-BASED DESIGN; HALOCYNTHIA-AURANTIUM; BIOACTIVE PEPTIDES; SOLITARY TUNICATE; DEGRADATION; STABILITY; ANALOGS; PURIFICATION; PROTEINASES;
D O I
10.1128/AAC.01790-09
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Cationic antimicrobial peptides (AMPs) have attracted a great deal of interest as a promising candidate for a novel class of antibiotics that might effectively treat recalcitrant infections caused by a variety of microbes that are resistant to currently available drugs. However, the AMPs are inherently limited in that they are inevitably susceptible to attacks by proteases generated by human and pathogenic microbes; this vulnerability severely hinders their pharmaceutical use in human therapeutic protocols. In this study, we report that a halocidin-derived AMP, designated HG1, was found to be resistant to proteolytic degradation. As a result of its unique structural features, HG1 proved capable of preserving its antimicrobial activity after incubation with trypsin, chymotrypsin, and human matrix metalloprotease 7 (MMP-7). Additionally, HG1 was observed to exhibit profound antimicrobial activity in the presence of fluid from human skin wounds or proteins extracted from the culture supernatants of Staphylococcus aureus and Pseudomonas aeruginosa. Greater understanding of the structural motifs of HG1 required for its protease resistance might provide feasible ways to solve the problems intrinsic to the development of an AMP-based antibiotic.
引用
收藏
页码:2855 / 2866
页数:12
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