Enhancement of ajmalicine production in Catharanthus roseus cell cultures with methyl jasmonate is dependent on timing and dosage of elicitation

被引:53
作者
Lee-Parsons, CWT
Ertürk, S
Tengtrakool, J
机构
[1] Northeastern Univ, Dept Chem Engn, Snell Engn Ctr 342, Boston, MA 02115 USA
[2] Genzyme Corp, Bioengn, Framingham, MA 01701 USA
基金
美国国家科学基金会;
关键词
ajmalicine; alkaloid; Catharanthus roseus; elicitation; methyl jasmonate;
D O I
10.1023/B:BILE.0000045825.37395.94
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The optimum growth stage for enhancing ajmalicine production in Catharanthus roseus cultures with methyl jasmonate (MJ) was after 6 d growth. MJ added at 10 or 100 mum on day 6 gave a maximum ajmalicine production of 10.2 mg 1(-1), a 300% increase over that of non-elicited cultures.
引用
收藏
页码:1595 / 1599
页数:5
相关论文
共 50 条
  • [21] Synergistic and additive influence of cyclodextrins and methyl jasmonate on the expression of the terpenoid indole alkaloid pathway genes and metabolites in Catharanthus roseus cell cultures
    Almagro, Lorena
    Gutierrez, Jorge
    Angeles Pedreno, Maria
    Sottomayor, Mariana
    PLANT CELL TISSUE AND ORGAN CULTURE, 2014, 119 (03) : 543 - 551
  • [22] Elicitation with methyl-jasmonate stimulates peruvoside production in cell suspension cultures of Thevetia peruviana
    Arias Zabala, Mario
    Angarita, Monica
    Restrepo, Juan M.
    Caicedo, Luis A.
    Perea, Margarita
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2010, 46 (03) : 233 - 238
  • [23] Elicitation with methyl-jasmonate stimulates peruvoside production in cell suspension cultures of Thevetia peruviana
    Mario Arias Zabala
    Mónica Angarita
    Juan M. Restrepo
    Luis A. Caicedo
    Margarita Perea
    In Vitro Cellular & Developmental Biology - Plant, 2010, 46 : 233 - 238
  • [24] Low Levels of Gibberellic Acid Control the Biosynthesis of Ajmalicine in Catharanthus roseus Cell Suspension Cultures
    Amini, Anica
    Glevarec, Gaelle
    Andreu, Francoise
    Rideau, Marc
    Creche, Joel
    PLANTA MEDICA, 2009, 75 (02) : 187 - 191
  • [25] Effects of elicitation on different metabolic pathways in Catharanthus roseus (L) G. Don cell suspension cultures
    Moreno, PRH
    Poulsen, C
    vanderHeijden, R
    Verpoorte, R
    ENZYME AND MICROBIAL TECHNOLOGY, 1996, 18 (02) : 99 - 107
  • [26] Increased production of naphthoquinones in Impatiens balsamina root cultures by elicitation with methyl jasmonate
    Sakunphueak, Athip
    Panichayupakaranant, Pharkphoom
    BIORESOURCE TECHNOLOGY, 2010, 101 (22) : 8777 - 8783
  • [27] THE NEGLIGIBLE ROLE OF CARBON-DIOXIDE AND ETHYLENE IN AJMALICINE PRODUCTION BY CATHARANTHUS-ROSEUS CELL-SUSPENSIONS
    SCHLATMANN, JE
    FONCK, E
    TENHOOPEN, HJG
    HEIJNEN, JJ
    PLANT CELL REPORTS, 1994, 14 (2-3) : 157 - 160
  • [28] Enhancement of vindoline and vinblastine production in suspension-cultured cells of Catharanthus roseus by artemisinic acid elicitation
    Liu, Jinwei
    Zhu, Jianhua
    Tang, Le
    Wen, Wei
    Lv, Shuangshuang
    Yu, Rongmin
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2014, 30 (01) : 175 - 180
  • [29] Improved alkaloid production in Catharanthus roseus suspension cell cultures by various chemicals
    Zhao, J
    Zhu, WH
    Hu, Q
    He, XW
    BIOTECHNOLOGY LETTERS, 2000, 22 (15) : 1221 - 1226
  • [30] Improvement of indole alkaloid production in Catharanthus roseus cell cultures by osmotic shock
    Zhao, J
    Zhu, WH
    Hu, Q
    Guo, YQ
    BIOTECHNOLOGY LETTERS, 2000, 22 (15) : 1227 - 1231