Mycotoxins co-contamination: Methodological aspects and biological relevance of combined toxicity studies

被引:238
作者
Alassane-Kpembi, Imourana [1 ,2 ]
Schatzmayr, Gerd [3 ]
Taranu, Ionelia [4 ]
Marin, Daniela [4 ]
Puel, Olivier [1 ]
Oswald, Isabelle Paule [1 ]
机构
[1] Univ Toulouse, Toxalim, Res Ctr Food Toxicol, INRA,ENVT,INP PURPAN,UPS, Toulouse, France
[2] Hop Instruct Armees Camp Guezo, Cotonou, Benin
[3] Biomin Res Ctr, Tulln, Austria
[4] Natl Inst Res & Dev Anim Biol & Nutr IBNA, Calea Bucuresti, Calea Bucuresti, Balotesti, Romania
关键词
Additivity; antagonism; multi-contamination; mycotoxins; synergy; toxicological interactions; KIDNEY PK15 CELLS; STATISTICALLY DESIGNED EXPERIMENTS; INTESTINAL EPITHELIAL-CELLS; MESSENGER-RNA EXPRESSION; IN-VITRO CYTOTOXICITY; FUSARIUM MYCOTOXINS; AFLATOXIN B-1; FUMONISIN B-1; OCHRATOXIN-A; CYCLOPIAZONIC ACID;
D O I
10.1080/10408398.2016.1140632
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Mycotoxins are secondary fungal metabolites produced mainly by Aspergillus, Penicillium, and Fusarium. As evidenced by large-scale surveys, humans and animals are simultaneously exposed to several mycotoxins. Simultaneous exposure could result in synergistic, additive or antagonistic effects. However, most toxicity studies addressed the effects of mycotoxins separately.We present the experimental designs and we discuss the conclusions drawn from in vitro experiments exploring toxicological interactions of mycotoxins.We report more than 80 publications related to mycotoxin interactions. The studies explored combinations involving the regulated groups of mycotoxins, especially aflatoxins, ochratoxins, fumonisins, zearalenone and trichothecenes, but also the emerging mycotoxins beauvericin and enniatins. Over 50 publications are based on the arithmetic model of additivity. Few studies used the factorial designs or the theoretical biology-based models of additivity. The latter approaches are gaining increased attention. These analyses allow determination of the type of interaction and, optionally, its magnitude. The type of interaction reported for mycotoxin combinations depended on several factors, in particular cell models and the tested dose ranges. However, synergy among Fusarium toxins was highlighted in several studies. This review indicates that well-addressed in vitro studies remain valuable tools for the screening of interactive potential in mycotoxin mixtures.
引用
收藏
页码:3489 / 3507
页数:19
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