Purification, crystallization and preliminary X-ray analysis of two nudix hydrolases from Deninococcus radiodurans

被引:4
作者
Holbrook, EL
Schulze-Gahmen, U
Buchko, GW
Ni, SS
Kennedy, MA
Holbrook, SR
机构
[1] Univ Calif Berkeley, Lawrence Berkeley Lab, Dept Biol Struct, Phys Biosci Div, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[3] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA
来源
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2003年 / 59卷
关键词
D O I
10.1107/S0907444903002671
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Two nudix hydrolases from Deinococcus radiodurans have been purified and crystallized. Diffraction data have been collected to 1.4 and 1.9 Angstrom resolution for DR1025 and DR0079, respectively. DR1025 belongs to space group P4(1)2(1)2/P4(3)2(1)2, with unit-cell parameters a = b = 53.2, c = 122.6 Angstrom (unit-cell volume 346 883 Angstrom(3), V-M = 2.5 Angstrom(3) Da(-1), solvent content 50.2%). DR0079 belongs to space group C222(1), with unit-cell parameters a = 34.1, b = 157.2, c = 126.5 Angstrom (unit-cell volume 677 308 Angstrom(3), V-M = 2.2 Angstrom(3) Da(-1), solvent content 44.0%). The calculated cell content of DR1025 indicates the presence of one molecule in the asymmetric unit. Dynamic light scattering and gel filtration suggest it to be a dimer in solution. The space group and unit-cell parameters of DR0079 indicate the presence of two molecules per asymmetric unit. Gel filtration and NMR spectroscopy suggest it to be a monomer in solution.
引用
收藏
页码:737 / 740
页数:4
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