Exopolysaccharides produced by Lactobacillus strains suppress HT-29 cell growth via induction of G0/G1 cell cycle arrest and apoptosis

被引：64
作者：
Di, Wei [1 ]
Zhang, Lanwei [1 ,2 ]
Yi, Huaxi [2 ]
Han, Xue [1 ]
Zhang, Yingchun [1 ]
Xin, Liang [1 ]
机构：
[1] Harbin Inst Technol, Sch Chem & Chem Engn, Dept Food Sci & Engn, 73 Huanghe Rd, Harbin 150090, Heilongjiang, Peoples R China
[2] Ocean Univ China, Coll Food Sci & Engn, Funct Dairy & Probiot Engn Lab, Qingdao 266003, Shandong, Peoples R China
来源：
ONCOLOGY LETTERS | 2018年 / 16卷 / 03期
基金：
中国国家自然科学基金;
关键词：
anti-proliferation; cell cycle; apoptosis; exopolysaccharides; HT-29; cell; Lactobacillus strains; COLON-CANCER CELLS; ANTITUMOR-ACTIVITY; PROBIOTIC STRAIN; SUBSP PARACASEI; CASEI; POLYSACCHARIDE; PLANTARUM; IMMUNOMODULATION; BIOACTIVITIES; PREBIOTICS;
D O I：
10.3892/ol.2018.9129
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
In the present study, the effects of exopolysaccharides (EPS) from nine Lactobacillus strains with a high degree of bio-activity on human colon cancer cell line HT-29 were studied. The extracellular polymeric substances from 4 strains, namely K11, M5, SB27 and x12, displayed desirable anti-proliferative activity against HT-29 cells. Crude and acidic EPS were purified from the 4 strains and the inhibitory effects were further investigated. The crude and acidic EPS from these 4 strains exerted anti-proliferation effects on HT-29 cells in a dose-dependent manner but were nontoxic to Vero cells. Treatment with EPS significantly induced G0/G1 cell cycle arrest and apoptosis of HT-29 cells. Hoechst 33258 staining of acidic EPS-treated HT-29 cells revealed different degrees of morphological changes within the nucleus and the formation of apoptotic bodies. Caspase-3 activity was markedly upregulated in HT-29 cells following treatment with acidic EPS. In addition, acidic EPS from the SB27 strain demonstrated the most robust inhibitory effect on HT-29 cells. The results of the present study suggest that as an inducer of apoptosis EPS has the potential to be applied in the treatment of colorectal cancer.
引用
收藏
页码：3577 / 3586
页数：10
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