A bioluminescent method for measuring thymidylate kinase activity suitable for high-throughput screening of inhibitor

被引：6

作者：

Hu, Chun-Mei ^{[1
]}

Chang, Zee-Fen ^{[1
]}

机构：

[1] Natl Taiwan Univ, Coll Med, Inst Biochem & Mol Biol, Taipei 10617, Taiwan

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 398卷 / 02期

关键词：

THYMIDINE MONOPHOSPHATE KINASE; MYCOBACTERIUM-TUBERCULOSIS; NUCLEOSIDE ANALOGS; DNA-SYNTHESIS; GENE; DESIGN; PURINE; GROWTH; CELLS;

D O I：

10.1016/j.ab.2009.12.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Blocking human thymidylate kinase (TMPK) function has a chemosensitization effect in anticancer treatment. However, a rapid and sensitive TMPK activity assay method suitable for inhibitor screening has been lacking. We have designed a luciferase-coupled TMPK assay in which luminescence emission is proportional to the magnitude of TMPK inhibition. The advantages of using this new method over the conventional nicotinamide adenine dinucleotide (reduced form, NADH)-coupling method in screening inhibitor include low cost, low limit in detecting inhibitory signal, more accurate, and devoid of interference due to compound absorbance at 340 nm. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：269 / 271

页数：3

共 50 条

[41] High-throughput screening
Nature Biotechnology, 2001, 19 (7) : 689 - 689
[42] High-throughput screening
Nature Biotechnology, 2005, 23 (6) : 755 - 755
[43] High-Throughput Screening
Wildey, Mary Jo
Haunso, Anders
Tudor, Matthew
Webb, Maria
Connick, Jonathan H.
ANNUAL REPORTS IN MEDICINAL CHEMISTRY, VOL 50: PLATFORM TECHNOLOGIES IN DRUG DISCOVERY AND VALIDATION, 2017, 50 : 149 - 195
[44] High-throughput screening
Nature, 2001, 413 (6855) : 549 - 550
[45] High-throughput screening
Rogers, MV
DRUG DISCOVERY TODAY, 1997, 2 (07) : 306 - 306
[46] Simple and efficient method for improving high-throughput screening and high-throughput docking results.
Glick, M
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2004, 227 : U914 - U914
[47] High-Throughput Screening Reveals New Glutaminase Inhibitor Molecules
Costa, Renna K. E.
Rodrigues, Camila T.
Campos, Jean C. H.
Paradela, Luciana S.
Dias, Marilia M.
da Silva, Bianca Novaes
de Valega Negrao, Cyro von Zuben
Goncalves, Kaliandra de Almeida
Ascencao, Carolline F. R.
Adamoski, Douglas
Mercaldi, Gustavo Fernando
Bastos, Alliny C. S.
Batista, Fernanda A. H.
Figueira, Ana Carolina
Cordeiro, Artur T.
Ambrosio, Andre L. B.
Guido, Rafael V. C.
Dias, Sandra M. G.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE, 2021, 4 (06) : 1849 - 1866
[48] Deploying Fluorescent Nucleoside Analogues for High-Throughput Inhibitor Screening
Seebald, Leah
Madec, Amael G. E.
Imperiali, Barbara
CHEMBIOCHEM, 2020, 21 (1-2) : 108 - 112
[49] A high-throughput screening assay for hydroxynitrile lyase activity
Andexer, Jennifer
Guterl, Jan-Karl
Pohl, Martina
Eggert, Thorsten
CHEMICAL COMMUNICATIONS, 2006, (40) : 4201 - 4203
[50] Automated high-throughput activity and stability screening of electrocatalysts
Jenewein, Ken J.
Akkoc, Gun D.
Kormanyos, Attila
Cherevko, Serhiy
CHEM CATALYSIS, 2022, 2 (10): : 2778 - 2794

← 1 2 3 4 5 →