Effect of Bacterial Media on the Evaluation of the Antibacterial Activity of a Biomaterial Containing Inorganic Antibacterial Reagents or Antibiotics

被引：20

作者：

Ando, Yoshiki ^{[1
,2
]}

Miyamoto, Hiroshi ^{[1
]}

Noda, Iwao ^{[2
]}

Miyaji, Fumiaki ^{[2
]}

Shimazaki, Takafumi ^{[3
]}

Yonekura, Yutaka ^{[3
]}

Miyazaki, Masaki ^{[3
]}

Mawatari, Masaaki ^{[3
]}

Hotokebuchi, Takao ^{[3
]}

机构：

[1] Saga Univ, Dept Pathol & Microbiol, Div Microbiol, Fac Med, Saga 8498501, Japan

[2] Japan Med Mat Corp, Res Dept, Yodogawa Ku, Osaka 5320003, Japan

[3] Saga Univ, Dept Orthopaed Surg, Fac Med, Saga 8498501, Japan

来源：

BIOCONTROL SCIENCE | 2010年 / 15卷 / 01期

关键词：

Antibacterial biomaterial; Antibacterial evaluation method; Infection; Bone Cement; SERUM SENSITIVITY; IN-VIVO; GENTAMICIN; INFECTION; RELEASE; HIP; IMPLANTS; SURGERY; ANKLE; FOOT;

D O I：

10.4265/bio.15.15

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Several studies have been performed to assess the effectivesness of the antibacterial coating of a biomaterial to reduce surgical site infection. However, evaluations of these materials are inconsistent, and therefore it is difficult to compare their antibacterial performance. In this study, we evaluated the influence of different media such as nutrient broth (NB), Mueller-Hinton broth (MHB) and fetal bovine serum (FBS) on the antibacterial activity of AgNO3- or gentamicin-added bone cement using a modified ISO 22196 standard to devise a method to evaluate the antibacterial activity of biomaterials in vitro. The antibacterial activity results against Staphylococcus aureus and Escherichia coli were different in each medium. The antibacterial activity of AgNO3 in FBS was lower than the other media, whereas the antibacterial activity of gentamicin in FBS was higher than in the other media. It was assumed that the fluctuating antibacterial activity was influenced by serum components. The results showed that the ISO 22196 antibacterial evaluation method is suitable to evaluate antibacterial biomaterials after modifying the medium to FBS.

引用

页码：15 / 19

页数：5

共 27 条

[1] Allograft bone in two-stage revision of the hip for infection - Is it safe?
Ammon, P
Stockley, I
[J]. JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME, 2004, 86B (07): : 962 - 965
[2] Molecular events associated with reactive oxygen species and cell cycle progression in mammalian cells
Boonstra, J
Post, JA
[J]. GENE, 2004, 337 : 1 - 13
[3] Studies on biodegradation and release of gentamicin sulphate from interpenetrating network hydrogels based on poly(acrylic acid) and gelatin: in vitro and in vivo
Changez, M
Koul, V
Krishna, B
Dinda, AK
Choudhary, V
[J]. BIOMATERIALS, 2004, 25 (01) : 139 - 146
[4] MECHANISM OF BACTERICIDAL ACTION OF AMINOGLYCOSIDES
DAVIS, BD
[J]. MICROBIOLOGICAL REVIEWS, 1987, 51 (03) : 341 - 350
[5] Metal ion release from metal implants
Hanawa, T
[J]. MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 2004, 24 (6-8): : 745 - 752
[6] Hostacká A, 1998, J BASIC MICROB, V38, P383, DOI 10.1002/(SICI)1521-4028(199811)38:5/6<383::AID-JOBM383>3.0.CO
[7] 2-J
[8] Illingworth BL, 1998, J HEART VALVE DIS, V7, P524
[9] *INT ORG STAND, 2007, INT STAND PLAST MEAS
[10] *JAP STAND ASS, 2000, JAP IND STAND ANT PR, P2801

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