Characteristics and antifungal activity of a chitin binding protein from Ginkgo biloba

被引：117

作者：

Huang, X ^{[1
]}

Xie, WJ ^{[1
]}

Gong, ZZ ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China

来源：

FEBS LETTERS | 2000年 / 478卷 / 1-2期

关键词：

antifungal peptide; chitin; membrane permeabilization; chitinase; Ginkgo biloba;

D O I：

10.1016/S0014-5793(00)01834-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An antifungal peptide from leaves of Ginkgo biloba, designated GAFF, has been isolated. Its molecular mass of 4244.0 Da was determined by mass spectrometry. The complete amino acid sequence,vas obtained from automated Edman degradation. GAFF exhibited antifungal activity towards Pellicularia sasakii Ito, Alternaria alternata (Fries) Keissler, Fusarium graminearum Schw. and Fusarium moniliforme. Its activities differed among various fungi. GAFF could also cause increased hyphal membrane permeabilization and a rapid alkalization of the medium when applied at 100 mu g/ml to Pellicularia sasakii Ito hyphae. The amino acid sequence of GAFF shows characteristics of the cysteine/glycine-rich chitin binding domain of many chitin binding proteins. The cysteine residues are well conserved. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：123 / 126

页数：4

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