Genomic Polymorphism Associated with the Emergence of Virulent Isolates of Mycobacterium bovis in the Nile Delta

被引:15
作者
Abdelaal, Hazem F. M. [1 ]
Spalink, Daniel [2 ]
Amer, Ali [3 ]
Steinberg, Howard [1 ]
Hashish, Emad A. [4 ]
Nasr, Essam A. [5 ]
Talaat, Adel M. [1 ]
机构
[1] Univ Wisconsin, Dept Pathobiol Sci, Madison, WI 53706 USA
[2] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX USA
[3] Anim Hlth Res Inst, Giza, Egypt
[4] Zagazig Univ, Fac Vet Med, Dept Clin Pathol, Zagazig, Egypt
[5] Vet Serum & Vaccine Res Inst, Bacterial Diagnost Res Dept TB, Cairo, Egypt
关键词
TUBERCULOSIS COMPLEX; DECONTAMINATION METHODS; TUBERCLE-BACILLI; CLONAL COMPLEX; MIRU-VNTRPLUS; UNITED-STATES; RISK-FACTORS; INFECTION; CATTLE; DIFFERENTIATION;
D O I
10.1038/s41598-019-48106-3
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Mycobacterium bovis is responsible for bovine tuberculosis in both animals and humans. Despite being one of the most important global zoonotic disease, data related to the ecology and pathogenicity of bovine tuberculosis is scarce, especially in developing countries. In this report, we examined the dynamics of M. bovis transmission among dairy cattle in the Nile Delta of Egypt. Animals belonging to 27 herds from 7 governorates were tested by the Single Intradermal Comparative Skin Tuberculin (SICST), as a preliminary screen for the presence of bovine tuberculosis. Positive SICST reactors were identified in 3% of the animals spread among 40% of the examined herds. Post-mortem examination of slaughtered reactors confirmed the presence of both pulmonary and/or digestive forms of tuberculosis in > 50% of the examined animals. Targeted and whole-genome analysis of M. bovis isolates indicated the emergences of a predominant spoligotype (SB0268) between 2013-2015, suggesting a recent clonal spread of this isolate within the Nile Delta. Surprisingly, 2 isolates belonged to M. bovis BCG group, which are not allowed for animal vaccination in Egypt, while the rest of isolates belonged to the virulent M. bovis clonal complex European 2 present in Latin America and several European countries. Analysis of strain virulence in the murine model of tuberculosis indicated the emergence of a more virulent strain (MBE4) with a specific genotype. More analysis is needed to understand the molecular basis for successful spread of virulent isolates of bovine tuberculosis among animals and to establish genotype/phenotype association.
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页数:15
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