Improved spatial resolution in fluorescence focal modulation microscopy

被引：28

作者：

Gong, Wei ^{[1
]}

Si, Ke ^{[2
]}

Chen, Nanguang ^{[1
,3
]}

Sheppard, Colin J. R. ^{[1
,2
,4
]}

机构：

[1] Natl Univ Singapore, Div Bioengn, Singapore 117576, Singapore

[2] Natl Univ Singapore, Grad Sch Integrat Sci & Engn, Singapore 117456, Singapore

[3] Natl Univ Singapore, Dept Elect & Comp Engn, Singapore 117576, Singapore

[4] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

来源：

OPTICS LETTERS | 2009年 / 34卷 / 22期

关键词：

D O I：

10.1364/OL.34.003508

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We show that the focal modulation microscopy (FMM), which combines a spatial phase modulator with confocal microscopy, results in an improvement in spatial resolution. This technique was introduced to increase imaging depth into tissue and rejection of background from a thick scattering object. A theory for image formation in FMM is presented, and the effects of detecting the in-phase modulated fluorescence signal are discussed. Compared with conventional confocal microscopy, the width of the point-spread function for the in-phase fluorescence signal is improved by 16.4%. When applied to saturable fluorescence, the half-width at half-maximum is improved by 33.6%, 50.0%, and 62.9%, at demodulation frequencies 2 omega, 4 omega, and 8 omega, respectively. (C) 2009 Optical Society of America

引用

页码：3508 / 3510

页数：3

共 10 条

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