Improved spatial resolution in fluorescence focal modulation microscopy

被引：28

作者：

Gong, Wei ^{[1
]}

Si, Ke ^{[2
]}

Chen, Nanguang ^{[1
,3
]}

Sheppard, Colin J. R. ^{[1
,2
,4
]}

机构：

[1] Natl Univ Singapore, Div Bioengn, Singapore 117576, Singapore

[2] Natl Univ Singapore, Grad Sch Integrat Sci & Engn, Singapore 117456, Singapore

[3] Natl Univ Singapore, Dept Elect & Comp Engn, Singapore 117576, Singapore

[4] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

来源：

OPTICS LETTERS | 2009年 / 34卷 / 22期

关键词：

D O I：

10.1364/OL.34.003508

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We show that the focal modulation microscopy (FMM), which combines a spatial phase modulator with confocal microscopy, results in an improvement in spatial resolution. This technique was introduced to increase imaging depth into tissue and rejection of background from a thick scattering object. A theory for image formation in FMM is presented, and the effects of detecting the in-phase modulated fluorescence signal are discussed. Compared with conventional confocal microscopy, the width of the point-spread function for the in-phase fluorescence signal is improved by 16.4%. When applied to saturable fluorescence, the half-width at half-maximum is improved by 33.6%, 50.0%, and 62.9%, at demodulation frequencies 2 omega, 4 omega, and 8 omega, respectively. (C) 2009 Optical Society of America

引用

页码：3508 / 3510

页数：3

共 10 条

[1] Focal modulation microscopy [J].

Chen, Nanguang ;

Wong, Chee-Howe ;

Sheppard, Colin J. R. .

OPTICS EXPRESS, 2008, 16 (23) :18764-18769

[2] Penetration depth of single-, two-, and three-photon fluorescence microscopic imaging through human cortex structures: Monte Carlo simulation [J].

Deng, XY ;

Gu, M .

APPLIED OPTICS, 2003, 42 (16) :3321-3329

[3] Confocal reflectance theta line scanning microscope for imaging human skin in vivo [J].

Dwyer, PJ ;

DiMarzio, CA ;

Zavislan, JM ;

Fox, WJ ;

Rajadhyaksha, M .

OPTICS LETTERS, 2006, 31 (07) :942-944

[4] High-resolution confocal microscopy by saturated excitation of fluorescence [J].

Fujita, Katsumasa ;

Kobayashi, Minoru ;

Kawano, Shogo ;

Yamanaka, Masahito ;

Kawata, Satoshi .

PHYSICAL REVIEW LETTERS, 2007, 99 (22)

[5] Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution [J].

Gustafsson, MGL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (37) :13081-13086

[6] METHOD OF TWO-DIMENSIONAL BIPOLAR INCOHERENT IMAGE-PROCESSING BY ACOUSTOOPTIC 2-PUPIL SYNTHESIS [J].

POON, TC .

OPTICS LETTERS, 1985, 10 (05) :197-199

[7] Far-field fluorescence nanoscopy of diamond color centers by ground state depletion [J].

Rittweger, E. ;

Wildanger, D. ;

Hell, S. W. .

EPL, 2009, 86 (01)

[8] The divided aperture technique for microscopy through scattering media [J].

Sheppard, Colin J. R. ;

Gong, Wei ;

Si, Ke .

OPTICS EXPRESS, 2008, 16 (21) :17031-17038

[9] Three-dimensional coherent transfer function for a confocal microscope with two D-shaped pupils [J].

Si, Ke ;

Gong, Wei ;

Sheppard, Colin J. R. .

APPLIED OPTICS, 2009, 48 (05) :810-817

[10] Improved rejection of multiply scattered photons in confocal microscopy using dual-axes architecture [J].

Wong, Larry K. ;

Mandella, Michael J. ;

Kino, Gordon S. ;

Wang, Thomas D. .

OPTICS LETTERS, 2007, 32 (12) :1674-1676

← 1 →